Attachment and growth of cultured fibroblast cells on silk protein matrices
Article first published online: 13 SEP 2004
Copyright © 1995 John Wiley & Sons, Inc.
Journal of Biomedical Materials Research
Volume 29, Issue 10, pages 1215–1221, October 1995
How to Cite
Minoura, N., Aiba, S.-I., Gotoh, Y., Tsukada, M. and Imai, Y. (1995), Attachment and growth of cultured fibroblast cells on silk protein matrices. J. Biomed. Mater. Res., 29: 1215–1221. doi: 10.1002/jbm.820291008
- Issue published online: 13 SEP 2004
- Article first published online: 13 SEP 2004
- Manuscript Accepted: 9 MAR 1995
- Manuscript Received: 3 OCT 1994
The attachment and growth of L-929 cells on films made of Bombyx mori silk proteins—fibroin and sericin and their mixtures—was studied by a cell culture method. Both cell attachment and growth were dependent on a minimum of around 90% sericin in the mixture. The results from electron micrography as well as from the DSC measurements supported the notion that the mixture of the two proteins fibroin and sericin has a phase-separated structure in the solid state. The observed minimum of sericin in the cell attachment and growth is thought to be a result of this phase-separated structure. Films of pure component proteins (i.e., 100% fibroin or sericin) exhibited as high a cell attachment and growth as collagen, a widely used mammalian cell culture substrate. However, a morphological study of the attached cells revealed that the cells attached to silk fibroin were extended and had a spindle shape, just like the cells attached to collagen, while the cells attached to the silk sericin had a different shape. It is concluded, therefore, that the attachment condition on silk fibroin is ideal for the viability, growth and function of the cells. © 1995 John Wiley & Sons, Inc.