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High density of immobilized galactose ligand enhances hepatocyte attachment and function

Authors

  • Chao Yin,

    1. Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597
    2. Laboratory of Biomedical Polymeric Materials, Department of Chemistry, Wuhan University, Wuhan 430072, China
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  • Lei Ying,

    1. Department of Chemical Engineering, National University of Singapore, Singapore 119260
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  • Peng-Chi Zhang,

    1. Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597
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  • Ren-Xi Zhuo,

    1. Laboratory of Biomedical Polymeric Materials, Department of Chemistry, Wuhan University, Wuhan 430072, China
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  • En-Tang Kang,

    1. Department of Chemical Engineering, National University of Singapore, Singapore 119260
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  • Kam W. Leong,

    Corresponding author
    1. Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597
    2. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Room 726, Ross Building, 720 Rutland Avenue, Baltimore, Maryland 21205
    • Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597
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  • Hai-Quan Mao

    Corresponding author
    1. Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597
    2. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Room 726, Ross Building, 720 Rutland Avenue, Baltimore, Maryland 21205
    • Tissue and Therapeutic Engineering Lab, Johns Hopkins Singapore, Level 5 Clinical Research Center, NUS, 10 Medical Drive, Singapore 117597
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Abstract

Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6′-aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm2 on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 × 105 cells/cm2, attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1093–1104, 2003

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