Three-dimensional collagen fiber remodeling by mesenchymal stem cells requires the integrin–matrix interaction

Authors

  • Ching-Fang Chang,

    1. Stem Cell Laboratory, Medical Research and Education, Veterans General Hospital-Taipei, 201, Sec. 2, Shih-Pai Road, 112 Taipei, Taiwan, Republic of China
    2. Institute of Anatomy and Cell Biology, National Yang-Ming University, 155, Sec. 2, Li-Nong St., 112 Taipei, Taiwan, Republic of China
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    • These authors contributed equally to this work.

  • Ming-Wei Lee,

    1. Institute of Biomedical Engineering, National Yang-Ming University, 155, Sec. 2, Li-Nong St., 112 Taipei, Taiwan, Republic of China
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    • These authors contributed equally to this work.

  • Pei-Yin Kuo,

    1. Stem Cell Laboratory, Medical Research and Education, Veterans General Hospital-Taipei, 201, Sec. 2, Shih-Pai Road, 112 Taipei, Taiwan, Republic of China
    2. Institute of Anatomy and Cell Biology, National Yang-Ming University, 155, Sec. 2, Li-Nong St., 112 Taipei, Taiwan, Republic of China
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  • Yng-Jiin Wang,

    1. Institute of Biomedical Engineering, National Yang-Ming University, 155, Sec. 2, Li-Nong St., 112 Taipei, Taiwan, Republic of China
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  • Yi-Hsien Tu,

    1. Institute of Biomedical Engineering, National Yang-Ming University, 155, Sec. 2, Li-Nong St., 112 Taipei, Taiwan, Republic of China
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  • Shih-Chieh Hung

    Corresponding author
    1. Stem Cell Laboratory, Medical Research and Education, Veterans General Hospital-Taipei, 201, Sec. 2, Shih-Pai Road, 112 Taipei, Taiwan, Republic of China
    2. Department of Orthopaedics and Traumatology, Veterans General Hospital-Taipei, 201, Sec. 2, Shih-Pai Road, 112 Taipei, Taiwan, Republic of China
    3. Insitute of Clinical Medicine, National Yang-Ming University 155, Sec. 2, Li-Nong St., 112 Taipei, Taiwan, Republic of China
    • Stem Cell Laboratory, Medical Research and Education, Veterans General Hospital-Taipei, 201, Sec. 2, Shih-Pai Road, 112 Taipei, Taiwan, Republic of China
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Abstract

Tissue engineering aiming to repair or regenerate damaged tissues necessitates fabricating three-dimensional biomaterial scaffolds with controlled porosity for delivering cells. To facilitate cell distribution, a strategy using stem cell-based fabrication of biomaterials was tested in type II collagen fibers. Human mesenchymal stem cells when delivered in type II collagen assembled and reorganized these matrices and differentiated into spherical chondrocytes with the synthesis of cartilage proteins. The cell-mediated assembly and reorganization of collagen fibers was not limitless and only restricted to an appropriate ratio of cell number and collagen amount. The blocking of α2 or β1-integrin function with specific antibodies significantly impeded the collagen-assembly effects. In vitro chondrogenesis or in vivo cartilage formation of human mesenchymal stem cells was also dependent on the interactions between cells and surrounding matrices. This method for three-dimensional fabricating collagen fibers may generally be applied to other biomaterials, when combined with surface modification or ligand addition for cell adhesion. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007

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