The influence of intrinsic coagulation pathway on blood platelets activation by oxidized cellulose

  1. P. Křížová1,*,
  2. L. Mášová1,
  3. J. Suttnar1,
  4. P. Salaj1,
  5. J.E. Dyr1,
  6. J. Homola2,
  7. M. Pecka3

Article first published online: 1 FEB 2007

DOI: 10.1002/jbm.a.31060

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 82A, Issue 2, pages 274–280, August 2007

Additional Information

How to Cite

Křížová, P., Mášová, L., Suttnar, J., Salaj, P., Dyr, J.E., Homola, J. and Pecka, M. (2007), The influence of intrinsic coagulation pathway on blood platelets activation by oxidized cellulose. J. Biomed. Mater. Res., 82A: 274–280. doi: 10.1002/jbm.a.31060

Author Information

  1. 1

    Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 00 Prague 2, Czech Republic

  2. 2

    Institute of Radio Engineering and Electronics, Chaberská 57, 182 51 Prague 8, Czech Republic

  3. 3

    Faculty Hospital UK, Sokolská 408, 500 05 Hradec Králové, Czech Republic

*Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 00 Prague 2, Czech Republic

Publication History

  1. Issue published online: 14 JUN 2007
  2. Article first published online: 1 FEB 2007
  3. Manuscript Accepted: 8 AUG 2006
  4. Manuscript Revised: 25 JUL 2006
  5. Manuscript Received: 11 JAN 2006

Funded by

  • Ministry of Health of the Czech Republic. Grant Number: MZd ČR 2373601

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Keywords:

  • cellulose;
  • blood platelets;
  • serotonin;
  • coagulation factor VIII, IX, XII, XIII

Abstract

Oxidized cellulose is an effective haemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the whole blood. As a marker of platelet activation and aggregation we used serotonin release reaction and turbidity changes in time. We found that oxidized cellulose did not activate washed platelets reconstituted in plasma-free medium or plasma-free medium with fibrinogen; no reduction of platelet count was observed. Serotonin release in platelet-rich plasma incubated with oxidized cellulose started in the range from 5 to 10 min. Serotonin release from platelets reconstituted in plasma deficient in either coagulation factor V, VIII, IX, or XII was delayed. Blood platelets activation by oxidized cellulose requires calcium ions present in dispersion of oxidized cellulose. Factor XIII deficiency had no influence on blood platelets activation by oxidized cellulose. Our results clearly indicate the significance of intrinsic coagulation pathway activation on blood platelets activation by oxidized cellulose and so indirectly on the haemostyptic effect of oxidized cellulose. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007

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