Characterization of biomimetic calcium phosphate on phosphorylated chitosan films

  1. B. M. Chesnutt1,
  2. Y. Yuan1,
  3. N. Brahmandam1,
  4. Y. Yang2,
  5. J. L. Ong2,
  6. W. O. Haggard1,
  7. J. D. Bumgardner1,*

Article first published online: 12 FEB 2007

DOI: 10.1002/jbm.a.31070

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 82A, Issue 2, pages 343–353, August 2007

Additional Information

How to Cite

Chesnutt, B. M., Yuan, Y., Brahmandam, N., Yang, Y., Ong, J. L., Haggard, W. O. and Bumgardner, J. D. (2007), Characterization of biomimetic calcium phosphate on phosphorylated chitosan films. J. Biomed. Mater. Res., 82A: 343–353. doi: 10.1002/jbm.a.31070

Author Information

  1. 1

    Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee 38152

  2. 2

    Department of Biomedical Engineering, University of Tennessee Health Science Center, Memphis, Tennessee 38163

*Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee 38152

Publication History

  1. Issue published online: 14 JUN 2007
  2. Article first published online: 12 FEB 2007
  3. Manuscript Accepted: 2 AUG 2006
  4. Manuscript Revised: 28 JUN 2006
  5. Manuscript Received: 28 MAR 2006

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Keywords:

  • chitosan;
  • calcium phosphate;
  • biomimetic;
  • degree of deacetylation;
  • composite

Abstract

This study examined the effect of chitosan degree of deacetylation (DDA), concentration of simulated body fluid (SBF), and mineralization time on the composition, structure, and crystallinity of calcium phosphate (CaP) biomimetically deposited on chitosan and on osteoblast cell growth. Phosphorylated chitosan films of 92.3%, 87.4%, and 80.6% DDA were soaked in SBF (1.0× or 1.5×) for 7, 14, or 21 days. Scanning electron microscopy revealed that CaP precipitated from 1.5× SBF had a porous, granular morphology; while the coatings precipitated in 1.0× SBF were smoother and more uniform. X-ray diffraction showed that films mineralized in 1.0× SBF were amorphous, while films mineralized in 1.5× SBF for 21 days exhibited crystalline peaks similar to hydroxyapatite, with the most crystalline peaks seen on 92.3% DDA chitosan. When mineralized films were placed in cell media for 14 days, more calcium phosphate precipitated onto all films, and the most calcium phosphate was found on 92.3% DDA films mineralized in 1.5× SBF. After seven days of osteoblast culture, there were approximately three times as many cells (based on DNA measurements, p < 0.05) on 92.3% DDA films soaked in 1.0× SBF for seven or 21 days than on 80.6% DDA films soaked in 1.0× SBF for any length of time or any films soaked in 1.5× SBF. The DDA of chitosan, concentration of SBF and mineralization time affect the structure of and biological response to chitosan/biomimetic CaP films, and these factors must be considered when designing new materials to be used in orthopaedic and dental/craniofacial implant applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007

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