In vitro growth and differentiated activities of human periodontal ligament fibroblasts cultured on salmon collagen gel

  1. Nobuhiro Nagai1,*,
  2. Kazuo Mori2,
  3. Yasuharu Satoh3,
  4. Noritaka Takahashi3,
  5. Shunji Yunoki4,
  6. Kenji Tajima3,
  7. Masanobu Munekata3

Article first published online: 12 FEB 2007

DOI: 10.1002/jbm.a.31110

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 82A, Issue 2, pages 395–402, August 2007

Additional Information

How to Cite

Nagai, N., Mori, K., Satoh, Y., Takahashi, N., Yunoki, S., Tajima, K. and Munekata, M. (2007), In vitro growth and differentiated activities of human periodontal ligament fibroblasts cultured on salmon collagen gel. J. Biomed. Mater. Res., 82A: 395–402. doi: 10.1002/jbm.a.31110

Author Information

  1. 1

    Creative Research Initiative “Sousei” (CRIS), Hokkaido University, N21-W10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan

  2. 2

    Ihara & Company Ltd., 3-263-23 Zenibako, Otaru, Hokkaido 047-0261, Japan

  3. 3

    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan

  4. 4

    National Institute for Materials Science, Namiki 1-1, Tsukuba 305-0044, Japan

*Creative Research Initiative “Sousei” (CRIS), Hokkaido University, N21-W10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan

Publication History

  1. Issue published online: 14 JUN 2007
  2. Article first published online: 12 FEB 2007
  3. Manuscript Accepted: 18 SEP 2006
  4. Manuscript Revised: 18 AUG 2006
  5. Manuscript Received: 19 JUL 2006

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Keywords:

  • salmon collagen;
  • periodontal ligament fibroblasts;
  • growth rate;
  • alkaline phophatase activity;
  • quantitative RT-PCR

Abstract

Marine-derived collagen is expected to be a much safer alternative to calf collagen, which in medical applications carries the risk of bovine spongiform encephalopathy. In this study, acid-soluble collagen was extracted from salmon skin and crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide during fibril formation to produce a crosslinked salmon collagen (SC) gel. The growth rates and the differentiated functions of human periodontal ligament fibroblasts (HPdLFs) cultured on the SC gel were investigated. Growth was faster on the SC gel than on porcine collagen (PC) gel. In addition, the HPdLFs cultured on the SC gel exhibited higher alkaline phosphatase (ALP) activity than those cultured on the PC gel. Quantitative RT-PCR revealed higher mRNA expression of type I collagen, ALP, and osteocalcin in the HPdLFs cultured on the SC gel. HPdLFs had a flat shape on the SC gel and a spindle shape on the PC gel, as revealed by observation with scanning electron microscopy and immunostaining with cytoskeletal protein and vinculin. The results showed that HPdLFs could grow and show highly differentiated activity on the SC gel as well as on the PC gel. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007

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