Effects on gingival cells of hydroxyapatite immobilized on poly(ethylene-co-vinyl alcohol)

  1. Kazuaki Matsumura,
  2. Suong-Hyu Hyon,
  3. Naoki Nakajima,
  4. Sadami Tsutsumi*

Article first published online: 1 FEB 2007

DOI: 10.1002/jbm.a.31128

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 82A, Issue 2, pages 288–295, August 2007

Additional Information

How to Cite

Matsumura, K., Hyon, S.-H., Nakajima, N. and Tsutsumi, S. (2007), Effects on gingival cells of hydroxyapatite immobilized on poly(ethylene-co-vinyl alcohol). J. Biomed. Mater. Res., 82A: 288–295. doi: 10.1002/jbm.a.31128

Author Information

  1. Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan

*Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan

Publication History

  1. Issue published online: 14 JUN 2007
  2. Article first published online: 1 FEB 2007
  3. Manuscript Accepted: 3 OCT 2006
  4. Manuscript Revised: 7 AUG 2006
  5. Manuscript Received: 2 MAY 2005

Funded by

  • Ministry of Education, Culture, Sports, Science and Technology, Japan. Grant Number: 13557165

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Keywords:

  • periodontium;
  • EVA;
  • hydroxyapatite;
  • regeneration;
  • implant

Abstract

Hydroxyapatite was immobilized on poly(ethylene-co-vinyl alcohol) (EVA) by alternate soaking in aqueous CaCl2 and Na2HPO4 solutions, followed by carboxyl groups introduction through ozone exposure in order to investigate the nature of the gingival cells, to control their proliferation and properties and to develop a highly organized hybrid implant possessing periodontium. Human gingival cells were cultured on the ozone-exposed EVA, collagen-immobilized EVA, hydroxyapatite-immobilized EVA, and a conventional tissue culture dish. Cell proliferation was highest on the tissue culture dish and lowest on the hydroxyapatite-immobilized EVA. The results of RT-PCR of gingival cells on hydroxyapatite-immobilized EVA shows that mRNAs expressed in bone and periodontal ligament were determined. Furthermore, alkaline phosphatase activity and ELISA assay revealed that gingival cells acquired the osteoblastic properties when cultured on hydroxyapatite-immobilized EVA, suggesting that the periodontium might be regenerated around implants using gingival cells. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007

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