Immobilization of homing peptide on magnetite nanoparticles and its specificity in vitro

Authors

  • Zhi-Feng Gan,

    1. Department of Physics, Center of Functional Nanomaterials and Devices, East China Normal University, North Zhongshan Road 3663, Shanghai 200062, People's Republic of China
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  • Ji-Sen Jiang,

    Corresponding author
    1. Department of Physics, Center of Functional Nanomaterials and Devices, East China Normal University, North Zhongshan Road 3663, Shanghai 200062, People's Republic of China
    • Department of Physics, Center of Functional Nanomaterials and Devices, East China Normal University, North Zhongshan Road 3663, Shanghai 200062, People's Republic of China
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  • Yong Yang,

    1. Department of Physics, Center of Functional Nanomaterials and Devices, East China Normal University, North Zhongshan Road 3663, Shanghai 200062, People's Republic of China
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  • Bing Du,

    1. School of Life Science, East China Normal University, Shanghai 200062, People's Republic of China
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  • Min Qian,

    1. School of Life Science, East China Normal University, Shanghai 200062, People's Republic of China
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  • Ping Zhang

    1. School of Life Science, East China Normal University, Shanghai 200062, People's Republic of China
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Abstract

As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell. Homing peptide labeled with green fluorescent protein (A54-GFP) was successfully immobilized on the surfaces of magnetic nanoparticles and characterized by Fourier transform infrared spectroscopy as well as fluorescence microscopy. The binding efficiency was analyzed by performing adsorption equilibrium and SDS-PAGE electrophoresis. Specific binding of the nanoparticles functionalized with A54-GFP to human hepatocellular carcinoma cells in vitro was visualized using fluorescence microscopy. The results demonstrated the specificity of A54-GFP-coated magnetic nanoparticle to tumor cell, pointing to its great potential in magnetic cell separation and purification, magnetic resonance imaging (MRI), magnetic hyperthermia, and drug targeting. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008

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