Alginate/lactose-modified chitosan hydrogels: A bioactive biomaterial for chondrocyte encapsulation

Authors

  • Eleonora Marsich,

    Corresponding author
    1. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
    • Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
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  • Massimiliano Borgogna,

    1. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
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  • Ivan Donati,

    1. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
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  • Pamela Mozetic,

    1. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
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  • Berit L. Strand,

    1. Institute of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Sælands v 6/8, N-7491 Trondheim, Norway
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  • Santiago Gomez Salvador,

    1. Department of Pathological Anatomy, Faculty of Medicine, University of Cadiz, Plaza del Falla 9, SP-11003 Cadiz, Spain
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  • Franco Vittur,

    1. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
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  • Sergio Paoletti

    1. Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Licio Giorgieri 1, I-34127 Trieste, Italy
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Abstract

A new bioactive scaffold was prepared from a binary polysaccharide mixture composed of a polyanion (alginate) and a polycation (a lactose-modified chitosan, chitlac). Its potential use for articular chondrocytes encapsulation and cartilage reconstructive surgery applications has been studied. The hydrogel combines the ability of alginate to act as a 3D supporting structure with the capability of the second component (chitlac) to provide interactions with porcine articular chondrocytes. Physico-chemical characterization of the scaffold was accomplished by gel kinetics and compression measurements and demonstrated that alginate-chitlac mixture (AC-mixture) hydrogels exhibit better mechanical properties when compared with sole alginate hydrogels. Furthermore, biochemical and biological studies showed that these 3D scaffolds are able to maintain chondrocyte phenotype and particularly to significantly stimulate and promote chondrocyte growth and proliferation. In conclusion, the present study can be considered as a first step towards an engineered, biologically active scaffold for chondrocyte in vitro cultivation, expansion, and cell delivery. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008

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