Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by hepatocyte growth factor

Authors

  • Takashi Hoshiba,

    1. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-Ku, Yokohama 226-8501, Japan
    2. Biomaterials Center, National Institute for Materials Science, Tsukuba 305-0044, Japan
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    • These authors contributed equally to this work.

  • Mai Wakejima,

    1. Graduate School of Medicine, Tottori University, Yonago 683-8503, Japan
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    • These authors contributed equally to this work.

  • Chong-Su Cho,

    1. School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea
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  • Goshi Shiota,

    1. Graduate School of Medicine, Tottori University, Yonago 683-8503, Japan
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  • Toshihiro Akaike

    Corresponding author
    1. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-Ku, Yokohama 226-8501, Japan
    • Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-Cho, Midori-Ku, Yokohama 226-8501, Japan
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Abstract

The roles of growth factors and extracellular matrices (ECMs) in regulation of hepatocyte behaviors are very important for the establishment of liver-tissue engineering. Especially, collaboration between growth factors and ECMs is a big concern for liver-tissue engineering. In this study, the hepatocyte responses by hepatocyte growth factor (HGF) were compared between natural ECMs and a synthetic galactose-carrying polymer: poly(N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide) (PVLA). Hepatocytes underwent proliferation on type I collagen- and fibronectin-coated surfaces in the presence of HGF, whereas hepatocytes formed spheroid on laminin-1-, PVLA-, and poly-L-lysine (PLL)-coated surfaces in the presence of HGF without the activation of proliferation. HGF accelerated ECM deposition, especially laminin-10/11, beneath the hepatocytes cultured on PVLA- and PLL-coated surfaces and the deposited laminin-10/11 activated integrin signaling to collaborate with HGF signaling. Therefore, the deposited ECM molecules should be focused to clear the mechanism of hepatocyte behaviors in the presence of HGF. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008

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