Get access

Nanocrystalline diamond: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures

Authors

  • M. Amaral,

    1. CICECO, Department of Ceramics and Glass Engineering, University of Aveiro, 3810-193 Aveiro, Portugal
    Search for more papers by this author
  • A. G. Dias,

    1. Faculdade de Engenharia da Universidade do Porto (FEUP), Secção de Materiais (DEMM), Rua Dr Roberto Frias, 4200-465 Porto, Portugal
    2. Instituto de Engenharia Biomédica (INEB), Laboratório de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal
    Search for more papers by this author
  • P. S. Gomes,

    1. Laboratório de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dentária, Universidade do Porto (FMDUP), Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal
    Search for more papers by this author
  • M. A. Lopes,

    1. Faculdade de Engenharia da Universidade do Porto (FEUP), Secção de Materiais (DEMM), Rua Dr Roberto Frias, 4200-465 Porto, Portugal
    2. Instituto de Engenharia Biomédica (INEB), Laboratório de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal
    Search for more papers by this author
  • R. F. Silva,

    Corresponding author
    1. CICECO, Department of Ceramics and Glass Engineering, University of Aveiro, 3810-193 Aveiro, Portugal
    • CICECO, Department of Ceramics and Glass Engineering, University of Aveiro, 3810-193 Aveiro, Portugal
    Search for more papers by this author
  • J. D. Santos,

    1. Faculdade de Engenharia da Universidade do Porto (FEUP), Secção de Materiais (DEMM), Rua Dr Roberto Frias, 4200-465 Porto, Portugal
    2. Instituto de Engenharia Biomédica (INEB), Laboratório de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal
    Search for more papers by this author
  • M. H. Fernandes

    1. Laboratório de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dentária, Universidade do Porto (FMDUP), Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal
    Search for more papers by this author

Abstract

Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell culture assays using MG63 osteoblast-like and human bone marrow cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) substrates for NCD coatings based on a previous demonstration of the superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008

Ancillary