A DNA transporter based on mesoporous silica nanospheres mediated with polycation poly(allylamine hydrochloride) coating on mesopore surface

Authors

  • Fei Qin,

    1. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China
    Search for more papers by this author
  • Yuchuan Zhou,

    1. Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China
    Search for more papers by this author
  • Jianlin Shi,

    Corresponding author
    1. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China
    • State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China
    Search for more papers by this author
  • Yonglian Zhang

    1. Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China
    Search for more papers by this author

Abstract

Mesoporous silica nanospheres (MPNSs) were synthesized by the sol–gel method and were investigated by X-ray diffraction, nitrogen sorption, and transmission electron microscopy. The analyses showed that the MPNSs were highly dispersed, with a diameter range of 50–250 nm. Further, the polycation poly(allylamine hydrochloride) was coated on silica MPNSs to form a positively charged surface and to facilitate the loading of DNA molecules. The composite was successfully used as a intercellular carrier of DNA molecules, and this was confirmed by agarose gel electrophoresis and confocal laser scanning microscopy. Evidently, this composite has potential to be used as a carrier for a DNA delivery system. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009

Ancillary