Bioactive interpenetrating polymer network hydrogels that support corneal epithelial wound healing

Authors

  • David Myung,

    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
    2. Department of Chemical Engineering, Stanford University, 381 North-South Mall, Stauffer III, Stanford, California 94305
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  • Nabeel Farooqui,

    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
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  • Luo Luo Zheng,

    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
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  • Wongun Koh,

    1. Department of Chemical Engineering, Yonsei University, 134 Shinchon-dong, Seodaemoon-ku, Seoul 120-749, South Korea
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  • Sarita Gupta,

    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
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  • Amit Bakri,

    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
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  • Jaan Noolandi,

    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
    2. Department of Chemical Engineering, Stanford University, 381 North-South Mall, Stauffer III, Stanford, California 94305
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  • Jennifer R. Cochran,

    1. Department of Bioengineering, Stanford University, 318 Campus Drive, Clark Center, Stanford, California 94305
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  • Curtis W. Frank,

    1. Department of Chemical Engineering, Stanford University, 381 North-South Mall, Stauffer III, Stanford, California 94305
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  • Christopher N. Ta

    Corresponding author
    1. Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
    • Department of Ophthalmology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5080
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Abstract

The development and characterization of collagen-coupled poly(ethylene glycol)/poly(acrylic acid) (PEG/PAA) interpenetrating polymer network hydrogels is described. Quantitative amino acid analysis and FITC-labeling of collagen were used to determine the amount and distribution of collagen on the surface of the hydrogels. The bioactivity of the coupled collagen was detected by a conformation-specific antibody and was found to vary with the concentration of collagen reacted to the photochemically functionalized hydrogel surfaces. A wound healing assay based on an organ culture model demonstrated that this bioactive surface supports epithelial wound closure over the hydrogel but at a decreased rate relative to sham wounds. Implantation of the hydrogel into the corneas of live rabbits demonstrated that epithelial cell migration is supported by the material, although the rate of migration and morphology of the epithelium were not normal. The results from the study will be used as a guide toward the optimization of bioactive hydrogels with promise in corneal implant applications such as a corneal onlay and an artificial cornea. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009

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