Preparation, characterization, in-vitro drug release and cellular uptake of poly(caprolactone) grafted dextran copolymeric nanoparticles loaded with anticancer drug

  1. P. Prabu1,
  2. Atul A. Chaudhari2,
  3. N. Dharmaraj3,
  4. M. S. Khil4,
  5. S. Y. Park2,
  6. H. Y. Kim4,*

Article first published online: 31 JUL 2008

DOI: 10.1002/jbm.a.32163

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 90A, Issue 4, pages 1128–1136, 15 September 2009

Additional Information

How to Cite

Prabu, P., Chaudhari, A. A., Dharmaraj, N., Khil, M. S., Park, S. Y. and Kim, H. Y. (2009), Preparation, characterization, in-vitro drug release and cellular uptake of poly(caprolactone) grafted dextran copolymeric nanoparticles loaded with anticancer drug. J. Biomed. Mater. Res., 90A: 1128–1136. doi: 10.1002/jbm.a.32163

Author Information

  1. 1

    Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea

  2. 2

    Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea

  3. 3

    Department of Chemistry, Bharathiar University, Coimbatore 641 046, India

  4. 4

    Department of Textile Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea

*Department of Textile Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea

Publication History

  1. Issue published online: 4 AUG 2009
  2. Article first published online: 31 JUL 2008
  3. Manuscript Accepted: 21 MAY 2008
  4. Manuscript Revised: 10 MAY 2008
  5. Manuscript Received: 27 DEC 2007

Funded by

  • MOEHRD, The Center for Health Care Technology Development
  • The Ministry of Commerce, Industry and Energy Department. Grant Number: 10028211

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Keywords:

  • biodegradable polymers;
  • cancer chemotherapy;
  • drug release;
  • nanoparticles;
  • polymeric drug carriers

Abstract

Biodegradable and biocompatible polymers that are engineered to nanostructures play a key role in providing solution for sustained chemotherapy. This study is focused on preparation, drug encapsulation efficiency, in-vitro drug release, in-vitro cellular uptake and cell viability of poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) formulation containing vinblastine as the anticancer drug. Drug-loaded PGD NPs were prepared by a modified oil/water emulsion method and characterized by laser light scattering, atomic force microscopy (AFM), and zeta potential. The drug encapsulation efficiency was determined spectrophotometrically and in-vitro drug release was estimated using dialysis bag. Breast cancer cell line (MCF-7) was used to image and measure the cellular uptake of fluorescent PGD NPs. Cancer cell viability was assessed by treating MCF-7 cells with vinblastine-loaded PGD NPs by crystal violet staining method. Result showed that the vinblastine-loaded PGD NPs were superior in properties such as drug encapsulation efficiency, the cellular uptake and the cancer cell mortality. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009

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