Differentiation of mesenchymal stem cells in chitosan scaffolds with double micro and macroporosity

  1. Dunia M. García Cruz1,2,*,
  2. M. Gomes3,4,
  3. Rui. L. Reis3,4,
  4. David Moratal2,
  5. Manuel Salmerón-Sánchez1,2,5,
  6. Jose L. Gómez Ribelles1,2,5,
  7. Joao F. Mano3,4

Article first published online: 5 OCT 2010

DOI: 10.1002/jbm.a.32906

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 95A, Issue 4, pages 1182–1193, 15 December 2010

Additional Information

How to Cite

García Cruz, D. M., Gomes, M., Reis, Rui. L., Moratal, D., Salmerón-Sánchez, M., Gómez Ribelles, J. L. and Mano, J. F. (2010), Differentiation of mesenchymal stem cells in chitosan scaffolds with double micro and macroporosity. J. Biomed. Mater. Res., 95A: 1182–1193. doi: 10.1002/jbm.a.32906

Author Information

  1. 1

    Centro de Investigación Príncipe Felipe, Autopista del Saler 16, Valencia 46013, Spain

  2. 2

    Centro de Biomateriales e Ingeniería Tisular, Universidad Politécnica de Valencia, Valencia 46022, Spain

  3. 3

    3B's Research Group (Biomaterials, Biodegradables and Biomimetics), Department of Polymer Engineering, University of Minho, Braga 4710-057, Portugal

  4. 4

    PT Government Associated Laboratory, IBB-Institute for Biotechnology and Bioengineering, Braga, Portugal

  5. 5

    CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Valencia, Spain

*Centro de Investigación Príncipe Felipe, Autopista del Saler 16, Valencia 46013, Spain

Publication History

  1. Issue published online: 4 NOV 2010
  2. Article first published online: 5 OCT 2010
  3. Manuscript Accepted: 25 MAY 2010
  4. Manuscript Revised: 22 APR 2010
  5. Manuscript Received: 7 JAN 2010

Funded by

  • Spanish Ministry of Education (including the FEDER financial support). Grant Number: MAT2007-66759-C03-01
  • Science and Innovation Ministry. Grant Number: PTA2008-0943-P
  • Generalitat Valenciana (Grups d'investigació Emergents). Grant Number: GV/2009/126
  • VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions, Instituto de Salud Carlos III (European Regional Development Fund)
  • FCT. Grant Number: PTDC/QUI/68804/2006

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Keywords:

  • chitosan;
  • macroporous scaffolds;
  • mesenchymal stem cells;
  • tissue engineering

Abstract

Bone Marrow mesenchymal stem cells can be induced to differentiate into osteoblasts to regenerate damaged bone tissue using tissue engineering techniques. In this study, we examine the use of chitosan scaffolds with double pore structure prepared by an innovative method that combines freeze gelation (that produces micropores) and particle leaching out technique (that produces interconnected spherical macropores) seeking to enhance the osteogenic differentiation of goat bone marrow stromal cells (GBMSCs). The double pore architecture of the scaffold was characterized by scanning electron microscopy (SEM), microcomputed tomography and confocal laser scanning microscopy. The obtained hierarchical pore structure allowed very efficient seeding of GBMSCs that are able to occupy the whole volume of the scaffold, showing good adhesion and proliferation. GBMSCs were differentiated into osteoblasts as indicated by alkaline phosphatase activity and osteocalcin expression. The results of this study demonstrate that chitosan scaffold may be promising biomaterial for bone regeneration. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

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