Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential

  1. Megan K. Proulx,
  2. Shawn P. Carey,
  3. Lisa M. DiTroia,
  4. Craig M. Jones,
  5. Michael Fakharzadeh,
  6. Jacques P. Guyette,
  7. Amanda L. Clement,
  8. Robert G. Orr,
  9. Marsha W. Rolle,
  10. George D. Pins,
  11. Glenn R. Gaudette*

Article first published online: 29 NOV 2010

DOI: 10.1002/jbm.a.32978

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 96A, Issue 2, pages 301–312, February 2011

Additional Information

How to Cite

Proulx, M. K., Carey, S. P., DiTroia, L. M., Jones, C. M., Fakharzadeh, M., Guyette, J. P., Clement, A. L., Orr, R. G., Rolle, M. W., Pins, G. D. and Gaudette, G. R. (2011), Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential. J. Biomed. Mater. Res., 96A: 301–312. doi: 10.1002/jbm.a.32978

Author Information

  1. Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609

*Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609

Publication History

  1. Issue published online: 17 DEC 2010
  2. Article first published online: 29 NOV 2010
  3. Manuscript Accepted: 2 SEP 2010
  4. Manuscript Revised: 31 AUG 2010
  5. Manuscript Received: 14 MAY 2010

Funded by

  • National Institutes of Health. Grant Number: R21HL093639
  • American Heart Association (Scientist Development Grant to GRG and Student Scholarship in Cardiovascular Disease and Stroke to MKP)
  • Worcester Polytechnic Institute (Faculty Advancement in Research award)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (9041K)

Keywords:

  • myocardium;
  • heart;
  • mesenchymal stem cells;
  • microthreads;
  • fibrin

Abstract

We developed a method to produce discrete fibrin microthreads, which can be seeded with human mesenchymal stem cells (hMSCs) and used as a suture to enhance the efficiency and localization of cell delivery. To assess the efficacy of fibrin microthreads to support hMSC attachment, proliferation, and survival, microthreads (100 μm diameter per microthread) were bundled together, seeded with 50,000 hMSCs for 2 h, and cultured for 5 days. Cell density on microthread bundles increased over time in culture to a maximum average density of 731 ± 101 cells/mm2 after 5 days. A LIVE/DEAD assay confirmed that the cells were viable, and Ki-67 staining verified hMSC proliferation. In addition, functional differentiation assays demonstrated that hMSCs cultured on microthreads retained their ability to differentiate into adipocytes and osteocytes. The results of this study demonstrate that fibrin microthreads support hMSC viability and proliferation, while maintaining their multipotency. We anticipate that these cell-seeded fibrin microthreads will serve as a platform technology to improve localized delivery and engraftment of viable cells to damaged tissue. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.

View Full Article (HTML) Get PDF (9041K)