Difference between dogs and rats with regard to osteoclast-like cells in calcium-deficient hydroxyapatite-induced osteoinduction
Version of Record online: 8 DEC 2010
Copyright © 2010 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 96A, Issue 2, pages 402–412, February 2011
How to Cite
Akiyama, N., Takemoto, M., Fujibayashi, S., Neo, M., Hirano, M. and Nakamura, T. (2011), Difference between dogs and rats with regard to osteoclast-like cells in calcium-deficient hydroxyapatite-induced osteoinduction. J. Biomed. Mater. Res., 96A: 402–412. doi: 10.1002/jbm.a.32995
- Issue online: 17 DEC 2010
- Version of Record online: 8 DEC 2010
- Manuscript Accepted: 4 OCT 2010
- Manuscript Revised: 6 SEP 2010
- Manuscript Received: 19 SEP 2008
- Japanese Ministry of Education, Science, Sports and Culture. Grant Number: 17591566
- multinucleated cell;
Material-induced osteoinduction is reported in comparatively large animals such as dogs and pigs; however, it does not often occur in small animals such as rodents. In this study, we implanted porous calcium-deficient hydroxyapatite (CDHA) in the dorsal muscles of dogs and rats and compared the two species, with emphasis on multinucleated cells, by using hematoxylin and eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, transmission electron microscope (TEM) observation, and reverse transcription-polymerase chain reaction (RT-PCR). In CDHA extracted from dogs, numerous TRAP-positive multinucleated cells were detected after 2 weeks and new bone formation was observed after 4 weeks. In contrast, in rats, only a small number of TRAP-positive cells were detected and no bone formation was observed within 6 weeks. CDHA was more degraded in dogs than in rats. TEM observation of the multinucleated cells in CDHA extracted from dogs after 3 weeks revealed osteoclast-like features such as ruffled borders. However, CDHA extracted from rats did not exhibit osteoclast-like features. RT-PCR evaluation showed that the expression of cathepsin K was higher in dogs than in rats. These results indicate that TRAP-positive cells might be one of the main factors responsible for the cross-species difference in material-induced osteoinduction. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2011.