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Biomaterials coated by dental pulp cells as substrate for neural stem cell differentiation

Authors

  • Jose Miguel Soria,

    Corresponding author
    1. Facultad Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avda Seminario sn. 46113 Moncada, Valencia, Spain
    • Departamento de Fisiología, Farmacología y Toxicología, Facultad Ciencias de la Salud, Universidad CEU- Cardenal Herrera, Avenida del Seminario, Moncada 46313, Valencia, Spain
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  • María Sancho-Tello,

    1. Facultad de Medicina y Odontología, Departamento de Patología, Universidad de Valencia, Avda. Blasco Ibáñez 15-17 46010 Valencia, Spain
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  • M. Angeles Garcia Esparza,

    1. Facultad Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avda Seminario sn. 46113 Moncada, Valencia, Spain
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  • Vicente Mirabet,

    1. Centro de Transfusiones, Conselleria de Sanitat, Generalitat Valenciana, Avenida del Cid, 65-acc. 46014 Valencia, Spain
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  • Jose Vicente Bagan,

    1. Facultad de Medicina y Odontología, Departamento de Estomatología, Universidad de Valencia, Avda. Blasco Ibáñez 15-17 46010 Valencia, Spain
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  • Manuel Monleón,

    1. Centro de Biomateriales e Ingeniería Tisular, Universidad Politécnica de Valencia, Camino de Vera s/n 46022, Valencia, Spain
    2. Centro de Investigación Príncipe Felipe, Avda. Autopista del Saler, 16. 46012 Valencia, Spain
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  • Carmen Carda

    1. Facultad de Medicina y Odontología, Departamento de Patología, Universidad de Valencia, Avda. Blasco Ibáñez 15-17 46010 Valencia, Spain
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Abstract

This study is focused on the development of an in vitro hybrid system, consisting in a polymeric biomaterial covered by a dental pulp cellular stroma that acts as a scaffold offering a neurotrophic support for the subsequent survival and differentiation of neural stem cells. In the first place, the behavior of dental pulp stroma on the polymeric biomaterial based on ethyl acrylate and hydroxy ethyl acrylate copolymer was studied. For this purpose, cells from normal human third molars were grown onto 0.5-mm-diameter biomaterial discs. After cell culture, quantification of neurotrophic factors generated by the stromal cells was performed by means of an ELISA assay. In the second place, survival and differentiation of adult murine neural stem cells on the polymeric biomaterials covered by dental pulp stromal cells was studied. The results show the capacity of dental pulp cells to uniformly coat the majority of the material's surface and to secrete neurotrophic factors that become crucial for a subsequent differentiation of neural stem cells. The use of stromal cells cultured on scaffolding biomaterials provides neurotrophic pumps that may suggest new criteria for the design of cell therapy experiments in animal models to assist the repair of lesions in Central Nervous System. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.

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