These authors contributed equally to this work.
Award Winner in the Young Investigator Category, 2011 Society for Biomaterials Annual Meeting and Exposition, Orlando, Florida, April 13–16, 2011
Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs†
Article first published online: 11 FEB 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 97A, Issue 1, pages 93–102, April 2011
How to Cite
Yanagawa, F., Kaji, H., Jang, Y.-H., Bae, H., Yanan, D., Fukuda, J., Qi, H. and Khademhosseini, A. (2011), Directed assembly of cell-laden microgels for building porous three-dimensional tissue constructs. J. Biomed. Mater. Res., 97A: 93–102. doi: 10.1002/jbm.a.33034
This article was published online on 11 February 2011. Subsequently, it was determined that the award heading had been omitted, and the correction was published online on 4 April 2011.
- Issue published online: 22 FEB 2011
- Article first published online: 11 FEB 2011
- Manuscript Accepted: 3 DEC 2010
- Manuscript Received: 29 NOV 2010
- National Institutes of Health. Grant Numbers: DE019024, HL092836, HL099073
- National Science Foundation CAREER award. Grant Number: DMR0847287
- Office of Naval Research Young Investigator award
- JSPS Fellowship for Research Abroad
- National Research Foundation of Korea, Korean Government. Grant Number: NRF-2009-352-D00107
- directed assembly;
- poly(ethylene glycol) diacrylate;
- sodium alginate;
- oxygen diffusion
The organization of cells within a well-defined microenvironment is important in generating the resulting tissue function. However, the cellular organization within biodegradable scaffolds often does not resemble those of native tissues. In this study, we present directed assembly of microgels to organize cells for building porous 3D tissue constructs. Cell-laden microgels were generated by molding photocrosslinkable polyethylene glycol diacrylate within a poly(dimethyl siloxane) stencil. The resulting microgels were subsequently packed as individual layers (1 mm in height) on a glass substrate by removing the excess prepolymer solution around the microgels. These clusters were crosslinked and stacked on one another to fabricate thick 3D constructs that were greater than 1 cm in width and 3 mm in thickness. To generate pores within the engineered structures, sodium alginate microgels were integrated in the engineered constructs and used as a sacrificial template. These pores may be potentially useful for fabricating a vascular network to supply oxygen and nutrients to the engineered tissue constructs. This simple and versatile building approach may be a useful tool for various 3D tissue culture and engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.