Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds

  1. Weibo Zhang1,
  2. Ivy Pruitt Ahluwalia1,
  3. Robert Literman1,†,
  4. David L. Kaplan2,
  5. Pamela C. Yelick1,*

Article first published online: 11 APR 2011

DOI: 10.1002/jbm.a.33062

Issue

Journal of Biomedical Materials Research Part A

Journal of Biomedical Materials Research Part A

Volume 97A, Issue 4, pages 414–422, 15 June 2011

Additional Information

How to Cite

Zhang, W., Ahluwalia, I. P., Literman, R., Kaplan, D. L. and Yelick, P. C. (2011), Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds. Journal of Biomedical Materials Research Part A, 97A: 414–422. doi: 10.1002/jbm.a.33062

Author Information

  1. 1

    Division of Craniofacial and Molecular Genetics, Department of Oral and Maxillofacial Pathology, Tufts University School of Dental Medicine, Boston, Massachusetts

  2. 2

    Department of Biomedical Engineering, Tufts University, Medford, Massachusetts

  1. †

    PhD Candidate, Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa

*Department of Oral and Maxillofacial Pathology, Tufts University, 136 Harrison Avenue, Room M824, Boston, MA 02111

Publication History

  1. Issue published online: 4 MAY 2011
  2. Article first published online: 11 APR 2011
  3. Manuscript Accepted: 5 JAN 2011
  4. Manuscript Revised: 4 JAN 2011
  5. Manuscript Received: 7 OCT 2010

Funded by

  • NIH/NIDCR. Grant Number: R01 DE016132
  • NIH. Grant Numbers: R01 EB003210, P41 EB002520

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Keywords:

  • silk scaffolds;
  • dental pulp cells;
  • dental tissue regeneration;
  • degradation

Abstract

Silk scaffolds have been successfully used for a variety of tissue engineering applications due to their biocompatibility, diverse physical characteristics, and ability to support cell attachment and proliferation. Our prior characterization of 4-day postnatal rat tooth bud cells grown on hexafluoro-2-propanol (HFIP) silk scaffolds showed that the silk scaffolds not only supported osteodentin formation, but also guided the size and shape of the formed osteodentin. In this study, interactions between human dental pulp cells and HFIP and aqueous based silk scaffolds were studied under both in vitro and in vivo conditions. Silk scaffold porosity and incorporation of RGD and DMP peptides were examined. We found that the degradation of aqueous based silk is much faster than HFIP based silk scaffolds. Also, HFIP based silk scaffolds supported the soft dental pulp formation better than the aqueous based silk scaffolds. No distinct hard tissue regeneration was found in any of the implants, with or without additional cells. We conclude that alternative silk scaffold materials, and hDSC pre-seeding cell treatments or sorting and enrichment methods, need to be considered for successful dental hard tissue regeneration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.

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