Development of bioactive photocrosslinkable fibrous hydrogels
Article first published online: 4 MAY 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 98A, Issue 2, pages 167–176, August 2011
How to Cite
Stephens-Altus, J. S., Sundelacruz, P., Rowland, M. L. and West, J. L. (2011), Development of bioactive photocrosslinkable fibrous hydrogels. J. Biomed. Mater. Res., 98A: 167–176. doi: 10.1002/jbm.a.33095
- Issue published online: 17 JUN 2011
- Article first published online: 4 MAY 2011
- Manuscript Accepted: 14 FEB 2011
- Manuscript Revised: 3 DEC 2010
- Manuscript Received: 10 JUN 2010
- NSF graduate fellowship program
- HHMI Professor's Program
- fibrous architecture
Three-dimensional (3D) fibrous hydrogels were fabricated by blending two photoactive polymers, poly(ethylene glycol) diacrylate (PEGDA) and poly(vinyl alcohol) (PVA), and the resulting solution was electrospun. PEGDA is a commonly used hydrogel material for tissue engineering applications since its interaction with cells can be tuned by crosslinking in a variety of bioactive molecules including peptides and proteins. The PVA in these materials aids in fiber formation and stabilizes the fibrous network when hydrated. The average dry fiber diameter in the hydrogels was 1.02 μm and upon swelling, the fiber diameter increased approximately six-fold. Fibers were stable under cell culture conditions for up to 5 days. The adhesive ligand, RGDS, was readily incorporated into the fibrous network via the conjugation of RGDS to PEG-monoacrylate which was then crosslinked with the fibers. The bioactivity of the fibrous hydrogels was compared with peptide-modified PEGDA-based hydrogels. The two hydrogel materials had similar cell adhesion and viability. Cell morphology on the fibrous hydrogels was dendritic showing a more in vivo like representation, as compared to spread cell morphology on the PEGDA gels. The ability to generate 3D fibrous architectures in hydrogel systems opens up new areas of investigation in cell-material interactions and tissue formation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.