How to cite this article: Rojbani H, Nyan M, Ohya K, Kasugai S. 2011. Evaluation of the osteoconductivity of α-tricalcium phosphate, β-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. J Biomed Mater Res Part A 2011:98A:488–498.
1552-4965/asset/olbannerleft.jpg?v=1&s=343c79177ba7ba4a14e83022a135a85fff60897a)
1552-4965/asset/olbannerright.jpg?v=1&s=692ae6cfb9620aaa8486b8867aa2cc1f34ef54b2)
Evaluation of the osteoconductivity of α-tricalcium phosphate, β-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect†
Article first published online: 16 JUN 2011
DOI: 10.1002/jbm.a.33117
Copyright © 2011 Wiley Periodicals, Inc.
Issue
Journal of Biomedical Materials Research Part A
Volume 98A, Issue 4, pages 488–498, 15 September 2011
Additional Information
How to Cite
Rojbani, H., Nyan, M., Ohya, K. and Kasugai, S. (2011), Evaluation of the osteoconductivity of α-tricalcium phosphate, β-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. J. Biomed. Mater. Res., 98A: 488–498. doi: 10.1002/jbm.a.33117
- †
Publication History
- Issue published online: 5 AUG 2011
- Article first published online: 16 JUN 2011
- Manuscript Accepted: 17 MAR 2011
- Manuscript Revised: 8 JAN 2011
- Manuscript Received: 7 SEP 2010
- Abstract
- Article
- References
- Cited By
Keywords:
- osteoconductivity;
- α-tricalcium phosphate;
- simvastatin;
- degradation
Abstract
The purpose of this study is to evaluate the osteoconductivity of three different bone substitute materials: α-tricalcium phosphate (α-TCP), (β-TCP), and hydroxyapatite (HA), combined with or without simvastatin, which is a cholesterol synthesis inhibitor stimulating BMP-2 expression in osteoblasts. We used 72 Wistar rats and prepared two calvarial bone defects of 5 mm diameter in each rat. Defects were filled with the particles of 500–750 μm diameter combined with or without simvastatin at 0.1 mg dose for each defect. In the control group, defects were left empty. Animals were divided into seven groups: α-TCP, β-TCP, HA, α-TCP with simvastatin, β-TCP with simvastatin, HA with simvastatin, and control. The animals were sacrificed at 6 and 8 weeks. The calvariae were dissected out and analyzed with micro CT. The specimens were evaluated histologically and histomorphometrically. In α-TCP group, the amount of newly formed bone was significantly more than both HA and control groups but not significantly yet more than β-TCP group. Degradation of α-TCP was prominent and β-TCP showed slower rate while HA showed the least degradation. Combining the materials with Simvastatin led to increasing in the amount of newly formed bone. These results confirmed that α-TCP, β-TCP, and HA are osteoconductive materials acting as space maintainer for bone formation and that combining these materials with simvastatin stimulates bone regeneration and it also affects degradability of α-TCP and β-TCP. Conclusively, α-TCP has the advantage of higher rate of degradation allowing the more bone formation and combining α-TCP with simvastatin enhances this property. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.