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Chondrogenic differentiation of adult mesenchymal stem cells and embryonic cells in collagen scaffolds

Authors

  • Karen K. Ng,

    1. Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    2. VA Boston Healthcare System, Boston, Massachusetts 02130
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  • Hemant S. Thatte,

    1. VA Boston Healthcare System, Boston, Massachusetts 02130
    2. Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115
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  • Myron Spector

    Corresponding author
    1. Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    2. VA Boston Healthcare System, Boston, Massachusetts 02130
    3. Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115
    • Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    Search for more papers by this author

  • How to cite this article: Ng KK, Thatte HS, Spector M. 2011. Chondrogenic differentiation of adult mesenchymal stem cells and embryonic cells in collagen scaffolds. J Biomed Mater Res Part A 2011:99A:275-282.

Abstract

Many cell types and cellular microenvironments have been explored for articular cartilage tissue engineering. We compared the potential of bone marrow-derived mesenchymal stem cells (MSCs) and P19 embryonic carcinoma cells (ECCs), a pluripotent derivative of embryonic stem cells (ESCs), for cartilage histogenesis in porous collagen scaffolds in vitro. We found that while both MSCs and ECCs express α-smooth muscle actin (α-SMA), only MSCs exhibit condensation and contraction necessary for cartilage histogenesis. Furthermore, histology confirmed that only MSCs exhibited sulfated glycosaminoglycans and collagen type II formation after 14 days in culture. We conclude that MSCs appear to be superior over ECCs for cartilage regeneration under particular culture conditions. The α-SMA-expressing ECCs may not have contracted due to the absence of actin unit polymerization or the absence of myosin molecules. Our observations may explain the absence of a contractile scar in fetal wound healing. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.

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