How to cite this article: Novajra G, Vitale-Brovarone C, Knowles JC, Maina G, Aina V, Ghigo D, Bergandi L. 2011. Effects of TiO2-containing phosphate glasses on solubility and in vitro biocompatibility. J Biomed Mater Res Part A 2011:99A:295-306.
Effects of TiO2-containing phosphate glasses on solubility and in vitro biocompatibility †
Article first published online: 19 AUG 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 99A, Issue 2, pages 295–306, November 2011
How to Cite
Novajra, G., Vitale-Brovarone, C., Knowles, J. C., Maina, G., Aina, V., Ghigo, D. and Bergandi, L. (2011), Effects of TiO2-containing phosphate glasses on solubility and in vitro biocompatibility . J. Biomed. Mater. Res., 99A: 295–306. doi: 10.1002/jbm.a.33186
- Issue published online: 20 SEP 2011
- Article first published online: 19 AUG 2011
- Manuscript Accepted: 20 MAY 2011
- Manuscript Revised: 1 APR 2011
- Manuscript Received: 13 SEP 2010
- WCU Program through the National Research Foundation of Korea (NRF), Ministry of Education, Science and Technology. Grant Number: R31-10069
- Regione Piemonte (Ricerca Sanitaria Finalizzata)
- phosphate glasses;
- titanium dioxide;
- human MG-63 osteoblasts
Phosphate-based glasses with different amounts of titanium dioxide (TiO2), having the following molar composition 50P2O5-30CaO-9Na2O-3SiO2-3MgO-(5-x)K2O-xTiO2, (where x = 0, 2.5, 5 mol %), were synthesised and characterized in terms of solubility (according to ISO 10993-14), and in vitro biocompatibility using human MG-63 osteoblast cells. Dissolution tests were carried out in Tris-HCl (pH 7.4) to simulate the physiological pH and in citric acid (pH 3.0) to simulate an acidic environment. The weight loss decreased with increasing TiO2 content, a process further enhanced in acidic medium. TiO2 reduced the pH changes usually caused by the dissolution products released. Cellular tests showed that all the glasses studied (0–5 mol % TiO2) and TiCl4, used to investigate the biocompatibility of titanium ions, did not produce cytotoxic effects on human MG-63 osteoblasts for up to 5 days in culture. On the basis of these results, we suggest that TiO2-containing phosphate glasses could be promising substrates for bone tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.