Get access

Effects of TiO2-containing phosphate glasses on solubility and in vitro biocompatibility

Authors

  • G. Novajra,

    1. Materials Science and Chemical Engineering Department, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy
    Search for more papers by this author
  • C. Vitale-Brovarone,

    Corresponding author
    1. Materials Science and Chemical Engineering Department, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy
    • Materials Science and Chemical Engineering Department, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy
    Search for more papers by this author
  • J. C. Knowles,

    1. Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK
    2. WCU Research Centre of Nanobiomedical Science, Dankook University, San#29, Anseo-dong, Dongnam-Gu, Cheonan-Si, Chungnam, 330-714, South Korea
    Search for more papers by this author
  • G. Maina,

    1. Traumatology Orthopaedics and Occupational Medicine Department, University of Torino, Via Zuretti 29, 10126 Torino, Italy
    Search for more papers by this author
  • V. Aina,

    1. Department of Chemistry IFM and Centre of Excellence NIS, University of Torino, Via Giuria 7, 10125 Torino, Italy
    Search for more papers by this author
  • D. Ghigo,

    1. Department of Genetics, Biology and Biochemistry, University of Torino, Via Santena 5/bis, 10126 Torino, Italy
    Search for more papers by this author
  • L. Bergandi

    1. Department of Genetics, Biology and Biochemistry, University of Torino, Via Santena 5/bis, 10126 Torino, Italy
    Search for more papers by this author

  • How to cite this article: Novajra G, Vitale-Brovarone C, Knowles JC, Maina G, Aina V, Ghigo D, Bergandi L. 2011. Effects of TiO2-containing phosphate glasses on solubility and in vitro biocompatibility. J Biomed Mater Res Part A 2011:99A:295-306.

Abstract

Phosphate-based glasses with different amounts of titanium dioxide (TiO2), having the following molar composition 50P2O5-30CaO-9Na2O-3SiO2-3MgO-(5-x)K2O-xTiO2, (where x = 0, 2.5, 5 mol %), were synthesised and characterized in terms of solubility (according to ISO 10993-14), and in vitro biocompatibility using human MG-63 osteoblast cells. Dissolution tests were carried out in Tris-HCl (pH 7.4) to simulate the physiological pH and in citric acid (pH 3.0) to simulate an acidic environment. The weight loss decreased with increasing TiO2 content, a process further enhanced in acidic medium. TiO2 reduced the pH changes usually caused by the dissolution products released. Cellular tests showed that all the glasses studied (0–5 mol % TiO2) and TiCl4, used to investigate the biocompatibility of titanium ions, did not produce cytotoxic effects on human MG-63 osteoblasts for up to 5 days in culture. On the basis of these results, we suggest that TiO2-containing phosphate glasses could be promising substrates for bone tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.

Get access to the full text of this article

Ancillary