How to cite this article: Hung H-S, Chu M-Y, Lin C-H, Wu C-C, Hsu S-h. 2012. Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin-focal adhesion kinase signaling. J Biomed Mater Res Part A 2012:100A:26–37.
Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin-focal adhesion kinase signaling †
Article first published online: 4 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 1, pages 26–37, January 2012
How to Cite
Hung, H.-S., Chu, M.-Y., Lin, C.-H., Wu, C.-C. and Hsu, S.-h. (2012), Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin-focal adhesion kinase signaling . J. Biomed. Mater. Res., 100A: 26–37. doi: 10.1002/jbm.a.33224
- Issue published online: 21 NOV 2011
- Article first published online: 4 OCT 2011
- Manuscript Accepted: 27 JUN 2011
- Manuscript Revised: 26 APR 2011
- Manuscript Received: 22 DEC 2010
- National Health Research Institutes, Taiwan
- China Medical University. Grant Number: CMU-98-N1-13
- polyurethane-gold nanocomposites;
- endothelial cell;
- focal adhesion kinase;
- alpha5/beta3 integrin;
Model surfaces of polyurethane-gold nanocomposites (PU-Au) were used to examine cell behavior on nanophase-segregated materials. Previously we showed that endothelial cell (EC) migration on these materials was modulated by the PI3K/Akt/eNOS pathway. The present study, investigated the expressions of alpha5/beta3 (α5β3) integrin, focal adhesion kinase (FAK), and other downstream signal molecules such as the Rho family and matrix metalloproteinases 2 (MMP-2) induced by the materials in two different cells, that is bovine arterial endothelial cells (BAEC) and human skin fibroblasts (HSF). Both cells proliferated better on the more phase-separated PU-Au 43.5 ppm than on the less phase-separated controls (PU and PU-Au 174 ppm). On PU-Au 43.5 ppm, BAEC compared to HSF had denser actin fibers and were more extended. BAEC became rounded with Y-27632 treatment and shrunk with LY294002 treatment. Treatment by inhibitors only caused slight changes in HSF. The migration distance of BAEC on PU-Au 43.5 ppm was greater than that of HSF, and was significantly reduced by LY294002 or Y-27632 but not SU-1498. The expressions of p-FAK, p-RhoA, p-Rac/Cdc42, MMP2, and α5β3 integrin induced by PU-Au 43.5 ppm were more pronounced in BAEC versus HSF. Further enhancement in MMP2 and α5β3 integrin expressions by FAK-GFP transfection was more remarkable for cells on PU-Au 43.5 ppm. Our findings suggested that the integrin α5β3/FAK pathway may be induced by nanophase-separated materials in both ECs and fibroblasts to promote their proliferation/migration, while the crosstalk between the PI3K/Akt/eNOS pathway and FAK/Rho-GTPase activation may account for the greater effect in ECs than in fibroblasts. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.