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Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin-focal adhesion kinase signaling

Authors

  • Huey-Shan Hung,

    1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, Republic of China
    2. Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan, Republic of China
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  • Mei-Yun Chu,

    1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, Republic of China
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  • Chien-Hsun Lin,

    1. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, Republic of China
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  • Chia-Ching Wu,

    1. Department of Cell Biology and Anatomy, School of Medicine, National Cheng, Kung University, Tainan, Taiwan, Republic of China
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  • Shan-hui Hsu

    Corresponding author
    1. Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, Republic of China
    2. Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China
    • Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan, Republic of China
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  • How to cite this article: Hung H-S, Chu M-Y, Lin C-H, Wu C-C, Hsu S-h. 2012. Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin-focal adhesion kinase signaling. J Biomed Mater Res Part A 2012:100A:26–37.

Abstract

Model surfaces of polyurethane-gold nanocomposites (PU-Au) were used to examine cell behavior on nanophase-segregated materials. Previously we showed that endothelial cell (EC) migration on these materials was modulated by the PI3K/Akt/eNOS pathway. The present study, investigated the expressions of alpha5/beta3 (α5β3) integrin, focal adhesion kinase (FAK), and other downstream signal molecules such as the Rho family and matrix metalloproteinases 2 (MMP-2) induced by the materials in two different cells, that is bovine arterial endothelial cells (BAEC) and human skin fibroblasts (HSF). Both cells proliferated better on the more phase-separated PU-Au 43.5 ppm than on the less phase-separated controls (PU and PU-Au 174 ppm). On PU-Au 43.5 ppm, BAEC compared to HSF had denser actin fibers and were more extended. BAEC became rounded with Y-27632 treatment and shrunk with LY294002 treatment. Treatment by inhibitors only caused slight changes in HSF. The migration distance of BAEC on PU-Au 43.5 ppm was greater than that of HSF, and was significantly reduced by LY294002 or Y-27632 but not SU-1498. The expressions of p-FAK, p-RhoA, p-Rac/Cdc42, MMP2, and α5β3 integrin induced by PU-Au 43.5 ppm were more pronounced in BAEC versus HSF. Further enhancement in MMP2 and α5β3 integrin expressions by FAK-GFP transfection was more remarkable for cells on PU-Au 43.5 ppm. Our findings suggested that the integrin α5β3/FAK pathway may be induced by nanophase-separated materials in both ECs and fibroblasts to promote their proliferation/migration, while the crosstalk between the PI3K/Akt/eNOS pathway and FAK/Rho-GTPase activation may account for the greater effect in ECs than in fibroblasts. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.

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