How to cite this article: Shen C-C, Yang Y-C, Liu B-S. 2012. Peripheral nerve repair of transplanted undifferentiated adipose tissuederived stem cells in a biodegradable reinforced nerve conduit. J Biomed Mater Res Part A 2012:100A:48–63.
Peripheral nerve repair of transplanted undifferentiated adipose tissue-derived stem cells in a biodegradable reinforced nerve conduit †
Article first published online: 4 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 1, pages 48–63, January 2012
How to Cite
Shen, C.-C., Yang, Y.-C. and Liu, B.-S. (2012), Peripheral nerve repair of transplanted undifferentiated adipose tissue-derived stem cells in a biodegradable reinforced nerve conduit . J. Biomed. Mater. Res., 100A: 48–63. doi: 10.1002/jbm.a.33227
- Issue published online: 21 NOV 2011
- Article first published online: 4 OCT 2011
- Manuscript Accepted: 16 AUG 2011
- Manuscript Revised: 4 JUL 2011
- Manuscript Received: 14 APR 2011
- Taichung Veterans General Hospital and Central Taiwan University of Science and Technology. Grant Numbers: TCVGH-1004905C, TCVGH-CTUST-1007703
- National Science Council of the Republic of China, Taiwan. Grant Number: NSC 98-2320-B-166-002-MY3
- biodegradable nerve conduit;
- adipose tissue-derived stem cells;
- tissue culture;
- tissue engineering;
- peripheral nerve regeneration
This study proposes a biodegradable nerve conduit containing genipin-cross-linked gelatin annexed with tricalcium phosphate ceramic particles (genipin-gelatin-tricalcium phosphate, GGT) in peripheral nerve regeneration. Firstly, cytotoxicity tests revealed that the GGT-extracts were not toxic, and promoted the proliferation and neuronal differentiation of adipose tissue-derived stem cells (ADSCs). Secondly, the GGT composite film effectively supported ADSCs attachment and growth. Additionally, the GGT substrate was biocompatible with the neonatal rat sciatic nerve and produced a beneficial effect on peripheral nerve repair through in vitro tissue culture. Finally, the experiments in this study confirmed the effectiveness of a GGT/ADSCs nerve conduit as a guidance channel for repairing a 10-mm gap in a rat sciatic nerve. Eight weeks after implantation, the mean recovery index of compound muscle action potentials (CMAPs) was significantly different between the GGT/ADSCs and autografts groups (p < 0.05), both of which were significantly superior to the GGT group (p < 0.05). Furthermore, walking track analysis also showed a significantly higher sciatic function index (SFI) score (p < 0.05) and better toe spreading development in the GGT/ADSCs group than in the autograft group. Histological observations and immunohistochemistry revealed that the morphology and distribution patterns of nerve fibers in the GGT/ADSCs nerve conduits were similar to those of the autografts. The GGT nerve conduit offers a better scaffold for the incorporation of seeding undifferentiated ADSCs, and opens a new avenue to replace autologous nerve grafts for the rapid regeneration of damaged peripheral nerve tissues and an improved approach to patient care. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.