How to cite this article: Zhao Y, Yang R, Liu D, Sun M, Zhou L, Wang Z, Wan Y. 2012. Starburst low-molecular weight polyethylenimine for efficient gene delivery. J Biomed Mater Res Part A 2012:100A:134–140.
Starburst low-molecular weight polyethylenimine for efficient gene delivery †
Version of Record online: 14 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 1, pages 134–140, January 2012
How to Cite
Zhao, Y., Yang, R., Liu, D., Sun, M., Zhou, L., Wang, Z. and Wan, Y. (2012), Starburst low-molecular weight polyethylenimine for efficient gene delivery . J. Biomed. Mater. Res., 100A: 134–140. doi: 10.1002/jbm.a.33250
- Issue online: 21 NOV 2011
- Version of Record online: 14 OCT 2011
- Manuscript Accepted: 30 AUG 2011
- Manuscript Revised: 23 AUG 2011
- Manuscript Received: 29 JUN 2011
- Application Foundation and Advanced Technology (Tianjin Research Program). Grant Number: 11JCZDJC20600
- National Natural Science Foundation of China. Grant Numbers: 30870397, 30970741
- gene delivery;
Low-molecular weight polyethylenimine (LMW PEI) shows the advantage of low-cytotoxicity, but has been inefficient in gene delivery as a consequence of the low-charge density. A number of previous studies employed the approach of crosslinking to solve this problem. In this study, a starburst LMW PEI gene vector has been developed. It has a polyamidoamine (PAMAM) core conjugated with a shell composed of LWM PEI and polyethylene glycol (PEG), that is PAMAM-PEI-PEG. Plasmid DNA (pEGFP-N1) and human cervix epithelial carcinoma (HeLa) cells were used in the study. The results showed that the starburst LMW PEI could effectively condense DNA at N/P above 5. The polyplexes had a size of about 500 nm and a nearly neutral surface because of the PEG shielding effect. This novel gene vector is able to maintain the low-cytotoxicity of LMW PEI, whereas its transfection efficiency was significantly improved. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.