Get access

Polyvinylidene fluoride for proliferation and preservation of bovine corneal endothelial cells by enhancing type IV collagen production and deposition

Authors

  • Tsung-Jen Wang,

    1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
    2. Department of Ophthalmology, Taipei Medical University Hospital, Taipei, Taiwan
    3. Department of Ophthalmology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
    4. Department of Ophthalmology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan
    Search for more papers by this author
  • I-Jong Wang,

    1. Department of Ophthalmology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan
    Search for more papers by this author
  • Yi-Hsin Chen,

    1. Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
    Search for more papers by this author
  • Jui-Nan Lu,

    1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
    Search for more papers by this author
  • Tai-Horng Young

    Corresponding author
    1. Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
    2. Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
    • Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
    Search for more papers by this author

  • How to cite this article: Wang T-J, Wang I-J, Chen Y-H, Lu J-N, Young T-H. 2012. Polyvinylidene fluoride for proliferation and preservation of bovine corneal endothelial cells by enhancing type IV collagen production and deposition. J Biomed Mater Res Part A 2012:100A:252–260.

Abstract

In this study, biomaterials with different hydrophobic properties including polyvinyl alcohol (PVA), poly(ethylene-co-vinyl alcohol) (EVAL), tissue culture polystyrene (TCPS), and polyvinylidene fluoride (PVDF) were examined in the bovine corneal endothelial cells (BCECs) culture system to elucidate their possible impact on clinical demand and scientific interest. It was found that BCECs were inhibited to attach onto the PVA surface. Conversely, relatively more hydrophobic biomaterials EVAL, TCPS, and PVDF successfully initiate BCEC adhesion. Compared to EVAL, cultured BCECs on TCPS and PVDF exhibited higher viability. Furthermore, fibroblastic transformation on EVAL and TCPS was observed at day 17, but BCECs maintained typical hexagonal shape on the PVDF surface at day 21. This phenomenon can be rescued by previously coating type IV collagen on TCPS but not on EVAL. In addition, when BCECs were cultured on PVDF, the expressions of gap junction connexin-43, differentiation marker N-cadherin, and tight junction ZO-1 were well-developed, resembling the physiological phenotypes. After examining the type IV collagen expression by Western blot analysis and protein absorption test, a possible explanation for the better proliferation and preservation of BCECs on the PVDF substrate is that PVDF is a bioactive substratum which enables BCECs to synthesize and reserve more extracellular matrix type IV collagen, paving an important way to provide a more preferential environment for BCEC cultures. Accordingly, promoting CEC growth effects after cell-biomaterial association may be applied to the tissue engineering of corneal endothelium. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

Ancillary