How to cite this article: Davydovskaya P, Janko M, Gaertner F, Ahmad Z, Simsek Ö, Maßberg S, Stark RW. 2012. Blood platelet adhesion to printed von Willebrand factor. J Biomed Mater Res Part A 2012:100A:335–341.
Blood platelet adhesion to printed von Willebrand factor†
Article first published online: 7 NOV 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 2, pages 335–341, February 2012
How to Cite
Davydovskaya, P., Janko, M., Gaertner, F., Ahmad, Z., Simsek, Ö., Maßberg, S. and Stark, R. W. (2012), Blood platelet adhesion to printed von Willebrand factor. J. Biomed. Mater. Res., 100A: 335–341. doi: 10.1002/jbm.a.33275
- Issue published online: 22 DEC 2011
- Article first published online: 7 NOV 2011
- Manuscript Revised: 12 SEP 2011
- Manuscript Accepted: 12 SEP 2011
- Manuscript Received: 15 JUN 2011
- Clusters of Excellence Center of Smart Interfaces Darmstadt
- Nanosystems Initiative Munich, NIM
- von Willebrand factor;
- atomic force microscope;
- micro contact printing;
- blood platelet adhesion;
- lab on a chip
Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.