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Blood platelet adhesion to printed von Willebrand factor

Authors

  • Polina Davydovskaya,

    1. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Theresienstraße 41, 80333 Munich, Germany
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  • Marek Janko,

    1. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Theresienstraße 41, 80333 Munich, Germany
    2. Center for NanoSciences, Ludwig-Maximilians-Universität München, Schellingstraße 4, 80799 Munich, Germany
    3. Center of Smart Interfaces, Technische Universität Darmstadt, Petersenstraße 32, 64287 Darmstadt, Germany
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  • Florian Gaertner,

    1. Deutsches Herzzentrum München, Technische Universität München, Lazarettstrasse 60, 80636 Munich, Germany
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  • Zerkah Ahmad,

    1. Deutsches Herzzentrum München, Technische Universität München, Lazarettstrasse 60, 80636 Munich, Germany
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  • Özlem Simsek,

    1. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Theresienstraße 41, 80333 Munich, Germany
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  • Steffen Maßberg,

    1. Deutsches Herzzentrum München, Technische Universität München, Lazarettstrasse 60, 80636 Munich, Germany
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  • Robert W. Stark

    Corresponding author
    1. Center for NanoSciences, Ludwig-Maximilians-Universität München, Schellingstraße 4, 80799 Munich, Germany
    2. Center of Smart Interfaces, Technische Universität Darmstadt, Petersenstraße 32, 64287 Darmstadt, Germany
    3. Material- und Geowissenschaften, Technische Universität Darmstadt, Petersenstraße 32, 64287 Darmstadt, Germany
    • Center for NanoSciences, Ludwig-Maximilians-Universität München, Schellingstraße 4, 80799 Munich, Germany
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  • How to cite this article: Davydovskaya P, Janko M, Gaertner F, Ahmad Z, Simsek Ö, Maßberg S, Stark RW. 2012. Blood platelet adhesion to printed von Willebrand factor. J Biomed Mater Res Part A 2012:100A:335–341.

Abstract

Von Willebrand factor (vWF), a glycoprotein in blood, mediates the adhesion of blood platelets and thus plays a crucial role in hemostasis and thrombosis. Functional coating of surfaces with vWF allows the investigation of in vitro adhesion of blood platelet. We used soft lithography to create a functional patterned substrate. vWF was printed on plasma-treated glass and mica surfaces, producing elongated network-like fibril structures. A minimum layer thickness of 3 nm was observed, corresponding to the height of a monolayer of vWF. The stability of the patterns was verified in a laminar fluid flow, and the bioactivity of the structures was tested with platelet adhesion experiments. Platelets adhered to and spread on printed vWF. These results indicate that printed vWF substrates are stable and functional in typical perfusion experiments, and thus provide a useful tool for studying thrombus formation in vitro. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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