Optimization of poly(ε-caprolactone) surface properties for apatite formation and improved osteogenic stimulation

Authors

  • Cleo Choong,

    Corresponding author
    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798
    • School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798
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  • Shaojun Yuan,

    1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798
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  • Eng San Thian,

    1. Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576
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  • Ayako Oyane,

    1. Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan
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  • James Triffitt

    1. Botnar Research Centre, Institute of Musculoskeletal Sciences, University of Oxford, Oxford OX3 7LD, United Kingdom
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  • How to cite this article: Choong C, Yuan S, Thian ES, Oyane A, Triffitt J. 2012. Optimization of poly(e-caprolactone) surface properties for apatite formation and improved osteogenic stimulation. J Biomed Mater Res Part A 2012:100A:353–361.

Abstract

A biodegradable polymer with surface properties that promotes cell attachment and host integration is widely recognized as a useful three-dimensional construct for bone tissue engineering applications. In this work, studies were carried out to correlate surface properties of modified polycaprolactone (PCL) films with cell-material interactions. PCL film substrates were subjected to various degrees of chemical hydrolysis using different pretreatment solutions to introduce different densities of carboxylate groups onto the surfaces. The extent of hydrolysis on the films was optimized to allow the deposition of a dense and uniform bone-like apatite layer by an alternate soak treatment, followed by subsequent incubation in simulated body fluid (SBF). The hydrolyzed and apatite-coated PCL films were investigated using scanning electron microscopy, thin film X-ray diffractometer (TF-XRD), water contact angle, and Alizarin red staining. Surface wettability, roughness, and chemistry of various PCL substrates were correlated with cell attachment, proliferation, viability, and alkaline phosphatase activity. Results demonstrated that cell attachment increased with increasing surface hydrophilicity and roughness. The apatite-coated films showed significantly improved surface wettability and enhanced surface roughness, which subsequently led to better cell attachment potential, high-cell viability, and enhanced bone formation capability. Thus, surface modification with an apatite coating layer is a promising approach for enhancing the efficacy of the polymeric scaffold for bone tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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