How to cite this article: Yamazoe H. 2012. Fabrication of protein micropatterns using a functional substrate with convertible protein-adsorption surface properties. J Biomed Mater Res Part A 2012:100A:362–369.
Fabrication of protein micropatterns using a functional substrate with convertible protein-adsorption surface properties†
Article first published online: 7 NOV 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 2, pages 362–369, February 2012
How to Cite
Yamazoe, H. (2012), Fabrication of protein micropatterns using a functional substrate with convertible protein-adsorption surface properties. J. Biomed. Mater. Res., 100A: 362–369. doi: 10.1002/jbm.a.33279
- Issue published online: 22 DEC 2011
- Article first published online: 7 NOV 2011
- Manuscript Accepted: 14 SEP 2011
- Manuscript Revised: 1 SEP 2011
- Manuscript Received: 9 JUN 2011
- Minister of Economy, Trade and Industry. Grant Number: 22130802099
- serum albumin;
- protein adsorption;
- inkjet printing;
A functional substrate capable of regulating protein adsorption was prepared using a crosslinked albumin (cl-albumin) film for use in the fabrication of protein micropatterns. The adsorption of proteins with different characteristics onto cl-albumin film, including serum proteins, serum albumin, and lysozyme, was investigated using a quartz crystal microbalance. The results showed that surfaces coated with cl-albumin film are highly resistant to protein adsorption, regardless of protein charge and rigidity. In addition, this adsorption-resistance property can be easily converted to promote protein adsorption by exposing the cl-albumin film to a charged polymer solution. By combining the convertible surface property of cl-albumin film and inkjet printing techniques, a precise protein micropattern was successfully fabricated on the substrate. Protein adsorption onto the wall surface of microchannels could also be suppressed or promoted by coating the surface with cl-albumin film. This approach will aid in the development of biomaterials carrying protein micropatterns, such as biosensors, biochips, and cellular scaffolds. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.