Fabrication of protein micropatterns using a functional substrate with convertible protein-adsorption surface properties

Authors

  • Hironori Yamazoe

    Corresponding author
    1. Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan
    • Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan
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  • How to cite this article: Yamazoe H. 2012. Fabrication of protein micropatterns using a functional substrate with convertible protein-adsorption surface properties. J Biomed Mater Res Part A 2012:100A:362–369.

Abstract

A functional substrate capable of regulating protein adsorption was prepared using a crosslinked albumin (cl-albumin) film for use in the fabrication of protein micropatterns. The adsorption of proteins with different characteristics onto cl-albumin film, including serum proteins, serum albumin, and lysozyme, was investigated using a quartz crystal microbalance. The results showed that surfaces coated with cl-albumin film are highly resistant to protein adsorption, regardless of protein charge and rigidity. In addition, this adsorption-resistance property can be easily converted to promote protein adsorption by exposing the cl-albumin film to a charged polymer solution. By combining the convertible surface property of cl-albumin film and inkjet printing techniques, a precise protein micropattern was successfully fabricated on the substrate. Protein adsorption onto the wall surface of microchannels could also be suppressed or promoted by coating the surface with cl-albumin film. This approach will aid in the development of biomaterials carrying protein micropatterns, such as biosensors, biochips, and cellular scaffolds. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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