Interactions between Schwann and olfactory ensheathing cells with a starch/polycaprolactone scaffold aimed at spinal cord injury repair

Authors

  • Nuno A. Silva,

    1. 3B's Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
    3. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
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  • Rui A. Sousa,

    1. 3B's Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
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  • Ana O. Pires,

    1. ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
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  • Nuno Sousa,

    1. ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
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  • António J. Salgado,

    Corresponding author
    1. ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
    2. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
    • ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
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    • These authors share senior authorship.

  • Rui L. Reis

    Corresponding author
    1. 3B's Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Guimarães, Portugal
    2. ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal
    • 3B's Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Guimarães, Portugal
    Search for more papers by this author
    • These authors share senior authorship.


  • How to cite this article: Silva NA, Sousa RA, Pires AO, Sousa N, Salgado AJ, Reis RL. 2012. Interactions between Schwann and olfactory ensheathing cells with a starch/polycaprolactone scaffold aimed at spinal cord injury repair. J Biomed Mater Res Part A 2012:100A:470–476.

Abstract

Spinal cord injury (SCI) represents a major world health problem. Therefore it is urgent to develop novel strategies that can specifically target it. We have previously shown that the implantation of starch-based scaffolds (SPCL) aimed for spine stabilization on SCI animals leads to motor skills improvements. Therefore, we hypothesize that the combination of these scaffolds with relevant cell populations for SCI repair will, most likely, lead to further improvements. Therefore, in this work, the ability of SPCL scaffolds to support the 3D culture of olfactory ensheathing cells (OECs) and Schwann cells (SCs) was studied and characterized. The results demonstrate for the first time that SPCL scaffolds were able to support the growth and migration of OECs and SCs. Moreover, the results indicate that two weeks of in vitro culture is the ideal time to reach a high number of transplantable cells. Future work will focus on the spine stabilization of SCI animals using SPCL scaffolds loaded with OECs or SCs for SCI regeneration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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