How to cite this article: Marino G, Rosso F, Ferdinando P, Grimaldi A, De Biasio G, Cafiero G, Barbarisi M, Barbarisi A. 2012. Growth and endothelial differentiation of adipose stem cells on polycaprolactone. J Biomed Mater Res Part A 2012:100A:543–548.
Growth and endothelial differentiation of adipose stem cells on polycaprolactone†
Version of Record online: 8 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 3, pages 543–548, March 2012
How to Cite
Marino, G., Rosso, F., Ferdinando, P., Grimaldi, A., De Biasio, G., Cafiero, G., Barbarisi, M. and Barbarisi, A. (2012), Growth and endothelial differentiation of adipose stem cells on polycaprolactone. J. Biomed. Mater. Res., 100A: 543–548. doi: 10.1002/jbm.a.33296
- Issue online: 24 JAN 2012
- Version of Record online: 8 DEC 2011
- Manuscript Accepted: 30 SEP 2011
- Manuscript Received: 29 SEP 2011
- stromal cells;
- human adipose stem cells;
- endothelial differentiation;
- vascular grafts;
- stem cells
Adipose tissue is a readily available source of multipotent adult stem cells for use in tissue engineering/regenerative medicine. Various growth factors have been used to stimulate acquisition of endothelial characteristics by adipose-derived stem cells (ADSC). Herein, we study the growth and endothelial differentiation potential of ADSC seeded onto a porous polycaprolactone (PCL) scaffold. The objective of this study is to demonstrate that PCL is a good material to be used as a scaffold to support reconstruction of new endothelial tissue using adipose stem cells. We found that undifferentiated ADSC adhere and grow on PCL. We show that, after culture in endothelial differentiation medium, ADSC were positive to LDL uptake and expressed molecular markers characteristic of endothelial cells (CD31; eNOS and vWF). In addition, our study defines the time required for the differentiation of ADSC directly onto PCL. This study suggests that PCL can be used as a scaffold to generate endothelial tissue in vitro. PLC has excellent mechanical properties and a slow degradation rate. Moreover, based on our results, we propose that PCL could be used to graft scaffolds coated with endothelial cells derived from ADSC stem cells. Endothelial cells-coated PCL could find several applications to replace damaged area of the body; for example, a possible use could be the generation of vascular grafts. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.