How to cite this article: Go DP, Palmer JA, Gras SL, O'Connor AJ. 2012. Coating and release of an anti-inflammatory hormone from PLGA microspheres for tissue engineering. J Biomed Mater Res Part A 2012:100A:507-517.
Coating and release of an anti-inflammatory hormone from PLGA microspheres for tissue engineering †
Version of Record online: 29 NOV 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 2, pages 507–517, February 2012
How to Cite
Go, D. P., Palmer, J. A., Gras, S. L. and O'Connor, A. J. (2012), Coating and release of an anti-inflammatory hormone from PLGA microspheres for tissue engineering . J. Biomed. Mater. Res., 100A: 507–517. doi: 10.1002/jbm.a.33299
- Issue online: 22 DEC 2011
- Version of Record online: 29 NOV 2011
- Manuscript Accepted: 6 OCT 2011
- Manuscript Revised: 5 AUG 2011
- Manuscript Received: 4 APR 2011
- foreign body reaction;
- surface modification
Many biomaterials used in tissue engineering cause a foreign body response in vivo, which left untreated can severely reduce the effectiveness of tissue regeneration. In this study, an anti-inflammatory hormone α-melanocyte stimulating hormone (α-MSH) was physically adsorbed to the surface of biodegradable poly (lactic-co-glycolic) acid (PLGA) microspheres to reduce inflammatory responses to this material. The stability and adsorption isotherm of peptide binding were characterized. The peptide secondary structure was not perturbed by the adsorption and subsequent desorption process. The α-MSH payload was released over 72 h and reduced the expression of the inflammatory cytokine, Tumor necrosis factor-α (TNF-α) in lipopolysaccharide activated RAW 264.7 macrophage cells, indicating that the biological activity of α-MSH was preserved. α-MSH coated PLGA microspheres also appeared to reduce the influx of inflammatory cells in a subcutaneous implantation model in rats. This study demonstrates the potential of α-MSH coatings for anti-inflammatory delivery and this approach may be applied to other tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.