How to cite this article: Wang G, Mostafa NZ, Incani V, Kucharski C, Uludaǧ H. 2012. Bisphosphonate-decorated lipid nanoparticles designed as drug carriers for bone diseases. J Biomed Mater Res Part A 2012:100A:684–693.
Bisphosphonate-decorated lipid nanoparticles designed as drug carriers for bone diseases †
Version of Record online: 30 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 3, pages 684–693, March 2012
How to Cite
Wang, G., Mostafa, N. Z., Incani, V., Kucharski, C. and Uludağ, H. (2012), Bisphosphonate-decorated lipid nanoparticles designed as drug carriers for bone diseases . J. Biomed. Mater. Res., 100A: 684–693. doi: 10.1002/jbm.a.34002
- Issue online: 24 JAN 2012
- Version of Record online: 30 DEC 2011
- Manuscript Accepted: 10 OCT 2011
- Manuscript Revised: 13 JUL 2011
- Manuscript Received: 9 DEC 2010
- Canadian Institutes of Health Research (CIHR)
- China Scholarship Council (CSC)
- CIHR-Skeletal Regenerative Medicine Team
- bone mineral affinity;
- drug delivery
A conjugate of distearoylphosphoethanolamine-polyethylene glycol with 2-(3-mercaptopropylsulfanyl)-ethyl-1,1-bisphosphonic acid (thiolBP) was synthesized and incorporated into micelles and liposomes to create mineral-binding nanocarriers for therapeutic agents. The micelles and liposomes were used to encapsulate the anticancer drug doxorubicin (DOX) and a model protein lysozyme (LYZ) by using lipid film hydration (LFH) and reverse-phase evaporation vesicle (REV) methods. The results indicated that the micelles and LFH-derived liposomes were better at DOX loading than the REV-derived liposomes, while the REV method was preferable for encapsulating LYZ. The affinity of the micellar and liposomal formulations to hydroxyapatite (HA) was assessed in vitro, and the results indicated that all the thiolBP-incorporated nanocarriers had stronger HA affinity than their counterparts without thiolBP. The thiolBP-decorated liposomes also displayed a strong binding to a collagen/HA composite scaffold in vitro. More importantly, thiolBP-decorated liposomes gave increased retention in the collagen/HA scaffolds after subcutaneously implantation in rats. The designed liposomes were able to entrap the bone morphogenetic protein-2 in a bioactive form, indicating that the proposed nanocarriers could deliver bioactive factors locally in mineralized scaffolds for bone tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.