Poly(dioxanone) (PDO) is colorless, crystalline, a biodegradable synthetic polymers that is used for biomedical applications, such as surgical sutures, cardiovascular applications, orthopedics, and plastic surgery. Recently, bone morphogenetic protein-2 (BMP-2) is widely used for bone tissue engineering. For the first time we report here on the in vitro performance of an electrospun PDO membrane immobilized with BMP-2. Immobilized BMP-2 on PDO membrane enhanced ALPase activity, the osteogenic differentiation gene expressions as well as cell attachment, except cell proliferation when compared to that of PDO membrane alone. These results suggest that PDO membrane with BMP-2 is helpful to promote bone healing and regeneration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.