Does the tissue engineering architecture of poly(3-hydroxybutyrate) scaffold affects cell–material interactions?

Authors

  • Elahe Masaeli,

    1. Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
    2. Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran
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  • Mohammad Morshed,

    Corresponding author
    1. Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
    • Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
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    • These authors contributed equally to this work.

  • Parsa Rasekhian,

    1. Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran
    2. Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan 81745-359, Iran
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  • Saeed Karbasi,

    Corresponding author
    1. Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan 15875-4413, Iran
    • Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan 15875-4413, Iran
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    • These authors contributed equally to this work.

  • Khadije Karbalaie,

    1. Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran
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  • Fereshte Karamali,

    1. Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran
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  • Daryoush Abedi,

    1. Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81745–359, Iran
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  • Shahnaz Razavi,

    1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81744–176, Iran
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  • Abbas Jafarian-Dehkordi,

    1. Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81745–359, Iran
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  • Mohammad Hossein Nasr-Esfahani,

    Corresponding author
    1. Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran
    • Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Cell Science Research Center, ACECR, Isfahan 81589-68433, Iran
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    • These authors contributed equally to this work.

  • Hossein Baharvand

    Corresponding author
    1. Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, Cell Science Research Center, ACECR, Tehran 19395-4644, Iran
    2. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran 13145-871, Iran
    • Department of Stem Cell and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran 19395-4644, Iran
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    • These authors contributed equally to this work.


  • How to cite this article: Masaeli E, Morshed M, Rasekhian P, Karbasi S, Karbalaie K, Karamali F, Abedi D, Razavi S, Jafarian-Dehkordi A, Nasr-Esfahani MH, Baharvand H. 2012. Does the tissue engineering architecture of poly(3-hydroxybutyrate) scaffold affects cell-material interactions? J Biomed Mater Res Part A 2012:100A:1907–1918.

Abstract

A critical element in tissue engineering involves the fabrication of a three-dimensional scaffold. The scaffold provides a space for new tissue formation, supports cellular ingrowth, and proliferation and mimics many roles of the extracellular matrix. Poly(3-hydroxybutyrate) (PHB) is the most thoroughly investigated member of the polyhydroxyalkanoates (PHAs) family that has various degrees of biocompatibility and biodegradability for tissue engineering applications. In this study, we fabricated PHB scaffolds by utilizing electrospinning and salt-leaching procedures. The behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) on these scaffolds was compared by the MTS assay and scanning electron microscopy. Additionally, this study investigated the mechanical and physical properties of these scaffolds by measuring tensile strength and modulus, dynamic contact angle and porosity. According to our results, the salt-leached scaffolds showed more wettability and permeability, but inferior mechanical properties when compared with nanofibrous scaffolds. In terms of cell response, salt-leached scaffolds showed enhanced Vero cell proliferation, whereas both scaffolds responded similarly in the case of mMSCs proliferation. In brief, nanofibrous scaffolds can be a better substrate for cell attachment and morphology. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.

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