How to cite this article: Grover CN, Farndale RW, Best SM, Cameron RE. 2012. The interplay between physical and chemical properties of protein films affects their bioactivity. J Biomed Mater Res Part A 2012:100A:2401–2411.
The interplay between physical and chemical properties of protein films affects their bioactivity†
Article first published online: 24 APR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 100A, Issue 9, pages 2401–2411, September 2012
How to Cite
Grover, C. N., Farndale, R. W., Best, S. M. and Cameron, R. E. (2012), The interplay between physical and chemical properties of protein films affects their bioactivity. J. Biomed. Mater. Res., 100A: 2401–2411. doi: 10.1002/jbm.a.34187
- Issue published online: 25 JUL 2012
- Article first published online: 24 APR 2012
- Manuscript Accepted: 15 MAR 2012
- Manuscript Received: 18 JAN 2012
- Engineering and Physical Sciences Research Council
- Medical Research Council
- myoblast cells;
Although mechanical properties, roughness, and receptor molecule expression have all been shown to influence the cellular reactivity of collagen-based biomaterials, their relative contribution, in a given system remains unclear. Here, we study films containing combinations of collagen, gelatin, and soluble and insoluble elastin, crosslinking of which results in altered film stiffness and roughness. Collagen and gelatin have similar amino acid sequences but altered cell-binding sites. We studied cell response with both C2C12 myoblast cells (which possess RGD-recognizing integrins αVβ3 and α5β1) and C2C12-α2+ cells (which, in addition, express the collagen-binding integrin α2β1) to establish the effect of altering the available binding sites on cell adhesion and spreading on films. Systematically altering the composition, crosslinking and cell type, allows us to deconvolute the effects of physical parameters and available binding sites on the cell reactivity of films in this system. Collagen-based films were rougher and stiffer and supported lower cell surface coverage than gelatin-based films. Additionally, C2C12-α2+ cells showed preferential attachment to collagen-based films compared with C2C12 cells, but no significant difference was seen using gelatin-based films. The cell count and surface coverage were found to decrease significantly on all films after crosslinking (Coll XL coverage = 2–6%, Gel XL coverage = 20–32%), but cell area and aspect ratio on collagen films were affected to a greater extent than on gelatin films. The results show that, in this system, the composition, and more significantly, crosslinking, of films affects the cell reactivity to a greater extent than their stiffness or roughness. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 2401–2411, 2012.