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Influence of the fetal bovine serum proteins on the growth of human osteoblast cells on graphene

Authors

  • Marie Kalbacova,

    1. Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University in Prague, Ke Karlovu 2, 12852 Prague, Czech Republic
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  • Antonin Broz,

    1. Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University in Prague, Ke Karlovu 2, 12852 Prague, Czech Republic
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  • Martin Kalbac

    Corresponding author
    1. J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of CR, Dolejskova 3, 18223 Prague 8, Czech Republic
    2. Department of Physical Chemistry, Palacky University, Olomouc 77146, Czech Republic
    • J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of CR, Dolejskova 3, 18223 Prague 8, Czech Republic
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  • How to cite this article: Kalbacova M, Broz A, Kalbac M. 2012. Influence of the fetal bovine serum proteins on the growth of human osteoblast cells on graphene. J Biomed Mater Res Part A 2012:100A:3001–3007.

Abstract

The influence of single-layer graphene produced by chemical vapor deposition on human osteoblast cells under different conditions was studied. Measurements probed the ability of cells to adhere and proliferate on graphene compared with SiO2/Si substrates and standard tissue culture plastic when cells were incubated for the first 2 h in the presence or the absence of fetal bovine serum (FBS), thus influencing the initial, direct interaction of cells with the substrate. It was found that after 48 h of human osteoblast incubation on graphene films, there were a comparable number of cells of a similar size irrespective of the presence or the absence of serum proteins. On the other hand, a strong initial influence through the presence of FBS proteins on cell number and cell size was observed in the case of the SiO2/Si substrate and control plastic. Thus, our study showed that the initial presence/absence of FBS in the medium does not determine cell fate in the case of a graphene substrate, which is very unusual and different from the behavior of cells on other materials. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3001–3007, 2012.

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