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Channeled scaffolds implanted in adult rat brain

Authors

  • Cristina Martínez-Ramos,

    1. Centro de Biomateriales, Universidad Politécnica de Valencia, PO Box 22012, E-46071 Valencia, Spain
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  • Ana Vallés-Lluch,

    1. Centro de Biomateriales, Universidad Politécnica de Valencia, PO Box 22012, E-46071 Valencia, Spain
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  • José Manuel García Verdugo,

    1. Instituto Cavanilles, Universidad de Valencia, Polígono La Coma, E-46980 Valencia, Spain
    2. CIBER de Enfermedades Neurodegenerativas, Instituto Nacional de Salud Carlos III, Spain
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  • José Luis Gómez Ribelles,

    1. Centro de Biomateriales, Universidad Politécnica de Valencia, PO Box 22012, E-46071 Valencia, Spain
    2. CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto Nacional de Salud Carlos III, Spain
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  • Juan Antonio Barcia Albacar,

    1. Hospital Clínico San Carlos, C/ Profesor Martín Lagos, S/N. Madrid, Spain
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    • *

      *These authors contributed equally to this work.

  • Amparo Baiget Orts,

    1. Centro de Biomateriales, Universidad Politécnica de Valencia, PO Box 22012, E-46071 Valencia, Spain
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  • José Miguel Soria López,

    1. CIBER de Enfermedades Neurodegenerativas, Instituto Nacional de Salud Carlos III, Spain
    2. Facultad Ciencias de la Salud, Universidad CEU Cardenal Herrera, Avda Seminario 46113 Moncada, Valencia, Spain
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    • *

      *These authors contributed equally to this work.

  • Manuel Monleón Pradas

    Corresponding author
    1. Centro de Biomateriales, Universidad Politécnica de Valencia, PO Box 22012, E-46071 Valencia, Spain
    2. CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto Nacional de Salud Carlos III, Spain
    • Centro de Biomateriales, Universidad Politécnica de Valencia, PO Box 22012, E-46071 Valencia, Spain

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  • How to cite this article: Martínez-Ramos C, Vallés-Lluch A, Verdugo JMG, Ribelles JLG, Barcia JA, Orts AB, López JMS, Pradas MM. 2012. Channeled scaffolds implanted in adult rat brain. J Biomed Mater Res Part A 2012:100A:3276–3286.

Abstract

Scaffolds with aligned channels based on acrylate copolymers, which had previously demonstrated good compatibility with neural progenitor cells were studied as colonizable structures both in vitro with neural progenitor cells and in vivo, implanted without cells in two different locations, in the cortical plate of adult rat brains and close to the subventricular zone. In vitro, neuroprogenitors colonize the scaffold and differentiate into neurons and glia within its channels. When implanted in vivo immunohistochemical analysis by confocal microscopy for neural and endothelial cells markers demonstrated that the scaffolds maintained continuity with the surrounding neural tissue and were colonized by GFAP-positive cells and, in the case of scaffolds implanted in contact with the subventricular zone, by neurons. Local angiogenesis was evidenced in the interior of the scaffolds' pores. New axons and neural cells from the adult neural niche abundantly colonized the biomaterial's inner structure after 2 months, and minimal scar formation was manifest around the implant. These findings indicate the biocompatibility of the polymeric material with the brain tissue and open possibilities to further studies on the relevance of factors such as scaffold structure, scaffold seeding and scaffold placement for their possible use in regenerative strategies in the central nervous system. The development of neural interfaces with minimized glial scar and improved tissue compatibility of the implants may also benefit from these results. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:3276–3286, 2012.

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