Osteocompatibility evaluation of poly(glycine ethyl ester-co-alanine ethyl ester)phosphazene with honeycomb-patterned surface topography

Authors

  • Shun Duan,

    1. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China
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  • Xiaoping Yang,

    1. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China
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  • Jifu Mao,

    1. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China
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  • Bing Qi,

    1. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China
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  • Qing Cai,

    Corresponding author
    1. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China
    • State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, North 3rd Ring Road, Chaoyang District, Beijing 100029, People's Republic of China
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  • Hong Shen,

    1. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Zhongguancun No. 1 Street, Haidian District, Beijing 100190, People's Republic of China
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  • Fei Yang,

    1. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Zhongguancun No. 1 Street, Haidian District, Beijing 100190, People's Republic of China
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  • Xuliang Deng,

    1. Department of Geriatric Dentistry, School and Hospital of Stomatology, Peking University, Zhongguancun South Road, Beijing 100081, People's Republic of China
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  • Shenguo Wang

    1. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Zhongguancun No. 1 Street, Haidian District, Beijing 100190, People's Republic of China
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  • How to cite this article: Duan S, Yang X, Mao J, Qi B, Cai Q, Shen H, Yang F, Deng X, Wang S. 2013. Osteocompatibility evaluation of poly(glycine ethyl ester-co-alanine ethyl ester)phosphazene with honeycomb-patterned surface topography. J Biomed Mater Res Part A 2012:101A:307–317.

  • This article was published online on 26 June 2012. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected on 3 December 2012.

Abstract

Biodegradable amino acid ester-substituted polyphosphazenes are unique biomaterials for tissue engineering. Considering the surface properties as topography and chemical composition having vital roles in regulating cellular response, in this study, a kind of micropatterned polyphosphazene films were prepared and subjected to osteoblasts culture. Briefly, poly(glycine ethyl ester-co-alanine ethyl ester)phosphazene (PGAP) was synthesized, and its solution in chloroform was cast under high (80%) or low (20%) environmental humidity. Honeycomb-patterned or flat PGAP films were resulted. By analyzing with scanning electron microscope, atomic force microscope, X-ray photoelectron spectroscope, and water contact angle measurement, the honeycomb-patterned PGAP films demonstrated higher surface roughness, phosphorous and nitrogen content, and hydrophilicity than the flat one. Although the initial cell attachment and proliferation on PGAP films were inferior to those on conventional poly(lactic-co-glycolic acid) films, P-containing PGAP was a sort of bone-binding bioactive polymer. With these alternations, honeycomb-patterned PGAP films had accordingly enhanced protein adsorption and apatite deposition in simulated body fluid and showed great advantages in promoting osteogenous differentiation. The results suggested a potential way to make polyphosphazenes as good choices for bone tissue regeneration by increasing their surface roughness and phosphorous content. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

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