Get access

Correlating macrophage morphology and cytokine production resulting from biomaterial contact

Authors

  • Hyun-Su Lee,

    1. Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania
    2. Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania
    3. Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for more papers by this author
  • Stanley J. Stachelek,

    1. Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania
    Search for more papers by this author
  • Nancy Tomczyk,

    1. Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for more papers by this author
  • Matthew J. Finley,

    1. Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania
    Search for more papers by this author
  • Russell J. Composto,

    1. Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania
    2. Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania
    Search for more papers by this author
  • David M. Eckmann

    Corresponding author
    1. Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania
    2. Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania
    • 331 John Morgan Building, 3620 Hamilton Walk, Philadelphia, PA 19104-6802
    Search for more papers by this author

  • How to cite this article: Lee H-S, Stachelek SJ, Tomczyk N, Finley MJ, Composto RJ, Eckmann DM. 2013. Correlating macrophage morphology and cytokine production resulting from biomaterial contact. J Biomed Mater Res Part A 2013:101A:203–212.

Abstract

The morphological and inflammatory responses of adherent macrophages are correlated to evaluate the biocompatibility of surfaces. Monocyte-derived macrophage (MDM), THP-1, and THP-1 cells expressing GFP-actin chimeric protein were seeded onto glass, polyurethane (PU), and glass surface modified with quaternary ammonium salt functionalized chitosan (CH-Q) and hyaluronic acid (HA). Using confocal microscopy, the surface area, volume and 3D shape factor of adherent macrophages was quantified. For comparison, functional consequences of cell-surface interactions that activate macrophages and thereby elicit secretion of a proinflammatory cytokine were evaluated. Using an enzyme linked immune sorbent assay, tumor necrosis factor-alpha (TNF-α) was measured. On glass, macrophages exhibited mainly an amoeboid shape, exhibited the largest surface area, volume, and 3D shape factor and produced the most TNF-α. On PU, macrophages displayed mainly a hemispherical shape, exhibited an intermediate volume, surface area and 3D shape factor, and produced moderate TNF-α. In contrast, on CH-Q and HA surfaces, macrophages were spherical, exhibited the smallest volume, surface area, and 3D shape factor, and produced the least TNF-α. These studies begin to validate the use of GFP-actin-modified MDM as a novel tool to correlate cell morphology with inflammatory cell response. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:203–212, 2013.

Ancillary