How to cite this article: Radu T, Chiriac MT, Popescu O, Simon V, Simon S. 2013. In vitro evaluation of the effects of yttria–alumina–silica microspheres on human keratinocyte cells. J Biomed Mater Res Part A 2013:101A:472–477.
In vitro evaluation of the effects of yttria–alumina–silica microspheres on human keratinocyte cells†
Article first published online: 10 AUG 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 472–477, February 2013
How to Cite
Radu, T., Chiriac, M. T., Popescu, O., Simon, V. and Simon, S. (2013), In vitro evaluation of the effects of yttria–alumina–silica microspheres on human keratinocyte cells. J. Biomed. Mater. Res., 101A: 472–477. doi: 10.1002/jbm.a.34320
- Issue published online: 18 DEC 2012
- Article first published online: 10 AUG 2012
- Manuscript Accepted: 15 JUN 2012
- Manuscript Revised: 29 MAY 2012
- Manuscript Received: 24 APR 2012
- Sectoral Operational Programme for Human Resources Development 2007–2013, cofinanced by the European Social Fund. Grant Number: POSDRU 89/1.5/S/60189 (Postdoctoral Programs for Sustainable Development in a Knowledge Based Society)
- PNII Idei PCCE-312/2008 project by the Romanian National University Research Council – CNCSIS Romania
- surface analysis;
The behavior of yttria–alumina–silica spray-dried microspheres was investigated in vitro on a human keratinocyte cell line, first to exclude their cytotoxicity. The HaCaT cells were chosen due to their well-characterized phenotype and their phagocytic ability. Microscopic analysis and cell viability tests showed no negative effect of the microspheres on cells morphology and behavior. Scanning electron microscopy and transmission electron microscopy results evidenced the cellular internalization of the microspheres at 48 h after their incubation with cultured cells. The shape, size distribution, structure, composition, and chemical states of the elements on samples surface were analyzed by SEM, transmission electron microscopy, x-ray diffraction, and x-ray photoelectron spectroscopy, because these properties could influence their internalization by cells. The yttrium distribution on the microspheres surface was indicated by fluorescence microscopy imaging. The microspheres dimension and shape inside the cells was in accordance with their dimension and shape before incubation. The microspheres seemed captured and engulfed by the cells in native form and appeared resistant to degradation over the first 48 h. Most of the analyzed cells took up more microspheres, suggesting that the microspheres were actively phagocytosed by the cells and accumulated within the cytoplasm. X-ray photoelectron spectroscopy results on Al and Si atomic environments denoted Al–O–Si crosslinks, which improve the surface protection to corrosion. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.