How to cite this article: Qu S, Bai Y, Liu X, Fu R, Duan K, Weng J. 2013. Study on in vitro release and cell response to alendronate sodium-loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles-induced osteolysis. J Biomed Mater Res Part A 2013:101A:394–403.
Study on in vitro release and cell response to alendronate sodium-loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles-induced osteolysis†
Article first published online: 31 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 394–403, February 2013
How to Cite
Qu, S., Bai, Y., Liu, X., Fu, R., Duan, K. and Weng, J. (2013), Study on in vitro release and cell response to alendronate sodium-loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles-induced osteolysis. J. Biomed. Mater. Res., 101A: 394–403. doi: 10.1002/jbm.a.34327
- Issue published online: 18 DEC 2012
- Article first published online: 31 JUL 2012
- Manuscript Accepted: 21 JUN 2012
- Manuscript Received: 26 JAN 2012
- National Basic Research Program of China (973 Program). Grant Number: 2012CB933602
- National Natural Science Foundation of China. Grant Number: 50975239
- Key Project of Chinese Ministry of Education. Grant Number: 109137
- Project of Sichuan Chengdu Science and Technology Bureau. Grant Number: 2102SZ0076
- Fundamental Research Funds for the Central Universities. Grant Numbers: SWJTU11CX118, SWJTU11ZT05
- alendronate sodium;
- wear debris;
- in vitro drug release;
- cell response
The aim of this study is to investigate in vitro release and cell response to wear particles of ultrahigh molecular weight polyethylene loaded with alendronate sodium (UHMWPE-ALN), a potent bone resorption inhibitor. Wear particles of UHMWPE-ALN with different ALN contents (0.5 wt % or 1.0 wt %) and size ranges (<45 μm or 45–75 μm) were cocultured with macrophages (RAW264.7) and osteoblasts (MC3T3-E1), respectively. The in vitro ALN release was divided into three stages: an initial burst release, subsequent rapid release, and final slow release. The particle size and ALN content of UHMWPE-ALN wear particles affected the in vitro release mainly during initial burst and rapid release. Compared with the control cells, UHMWPE-ALN wear particles stimulated a significant elevation of tumor necrosis factor-alpha (TNF-α) release from macrophages but had no obvious effect on interleukin-6 release. However, this stimulation of TNF-α release could be reduced by ALN released from UHMWPE-ALN wear particles. The wear particle size had stronger effect of on the macrophages compared with the ALN concentration. After coculture with UHMWPE-ALN wear particles, osteoblast proliferation and alkaline phosphatase activities increased moderately with the increase in particle sizes and ALN concentrations. These results suggest that incorporation of ALN in UHMWPE-ALN may be an effective approach to prevent or reduce particles-induced osteolysis. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.