How to cite this article: Baxter RM, Dai T, Kimball J, Wang E, Hamblin MR, Wiesmann WP, McCarthy SJ, Baker SM. 2013. Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling. J Biomed Mater Res Part A 2013:101A:340–348.
Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling†
Article first published online: 30 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 340–348, February 2013
How to Cite
Baxter, R. M., Dai, T., Kimball, J., Wang, E., Hamblin, M. R., Wiesmann, W. P., McCarthy, S. J. and Baker, S. M. (2013), Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling. J. Biomed. Mater. Res., 101A: 340–348. doi: 10.1002/jbm.a.34328
- Issue published online: 18 DEC 2012
- Article first published online: 30 JUL 2012
- Manuscript Accepted: 20 JUN 2012
- Manuscript Revised: 5 JUN 2012
- Manuscript Received: 4 JAN 2012
- NIH. Grant Number: R01AI050875
- Airlift Research Foundation Extremity Trauma Research Grant (Tianhong Dai). Grant Number: 109421
- US Army MRMC (Research at HemCon and Synedgen). Grant Number: W81XWH-08-2-0120
- wound healing;
- expression profiling;
Burns are a significant health challenge and healing can result in scar formation. Chitosan, a derivative of chitin, has been used to promote wound healing. In this study we used gene expression profiling in a mouse model of full thickness cutaneous burn to assess the benefits of treating with a chitosan lactate dressing. Three days after wounding mice treated with chitosan showed increased expression of genes associated with formation of granulation tissue. At a later time point, seven days after wounding, genes that initially showed increased expression were now down-regulated, and there was increased expression of genes involved in remodeling suggesting that the chitosan treatment results in accelerated healing. Quantitative RT-PCR showed modulated mRNA levels for TGFβ1 by the chitosan dressing. TGFβ1 initially promotes healing but extended activity can result in scarring. Importantly we found that expression was elevated at day three, but decreased at day seven suggesting that chitosan treatment will not result in scar formation, and may even be beneficial in preventing scar formation. Additionally, the biphasic regulation of expression of TGFβ1 could be a powerful biomarker for future studies of the wound-healing potential of chitosan based and other treatments for burn wounds. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.