How to cite this article: Gamboa-Martínez TC, García Cruz DM, Carda C, Gómez Ribelles JL, Gallego Ferrer G. 2013. Fibrin-chitosan composite substrate for in vitro culture of chondrocytes. J Biomed Mater Res Part A 2013:101A:404–412.
Fibrin-chitosan composite substrate for in vitro culture of chondrocytes †
Article first published online: 3 AUG 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 404–412, February 2013
How to Cite
Gamboa-Martínez, T. C., García Cruz, D. M., Carda, C., Ribelles, J. L. G. and Ferrer, G. G. (2013), Fibrin-chitosan composite substrate for in vitro culture of chondrocytes . J. Biomed. Mater. Res., 101A: 404–412. doi: 10.1002/jbm.a.34330
- Issue published online: 18 DEC 2012
- Article first published online: 3 AUG 2012
- Manuscript Accepted: 20 JUN 2012
- Manuscript Revised: 14 JUN 2012
- Manuscript Received: 12 MAR 2012
- Spanish Ministry. Grant Number: DPI2010-20399-C04-03
- chondrocyte redifferentiation
The aim of this study was to develop a biocompatible monolayer substrate based on fibrin and chitosan for in vitro culture of chondrocytes. Fibrin-chitosan composite substrates combined the proved cell adhesion properties of fibrin with the hydrophilicity and poor adhesion capacity of chitosan. Chitosan microspheres were produced by coacervation method, agglomerated within a fibrin network and subsequently crosslinked with genipin. The composite substrate was stable for 28 days of culture due to the high crosslinking density. Human chondrocytes cultured on the composite substrate were viable during the culture period. At the end of culture time (28 days) the composite substrate showed low cellular proliferation, 41% more collagen type II and 13% more production of sulfated glycosaminoglycans with respect to the amounts found at 14 days. The study revealed that dedifferentiated chondrocytes cultured in monolayer on the composite substrate can re-acquire characteristics of differentiated cells without using three-dimensional substrates or chondrogenic media. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.