These authors contributed equally to this work.
Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility †
Article first published online: 3 AUG 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part A
Volume 101A, Issue 2, pages 413–420, February 2013
How to Cite
Wang, H. G., Yin, T. Y., Ge, S. P., Zhang, Q., Dong, Q. L., Lei, D. X., Sun, D. M. and Wang, G. X. (2013), Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility . J. Biomed. Mater. Res., 101A: 413–420. doi: 10.1002/jbm.a.34339
How to cite this article: Wang HG, Yin TY, Ge SP, Zhang Q, Dong QL, Lei DX, Sun DM, Wang GX. 2013. Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility. J Biomed Mater Res Part A 2013:101A:413–420.
- Issue published online: 18 DEC 2012
- Article first published online: 3 AUG 2012
- Manuscript Accepted: 15 JUN 2012
- Manuscript Revised: 9 APR 2012
- Manuscript Received: 18 DEC 2011
- National Key Technology R & D Program of China. Grant Number: 2012 BAI18B02
- National Key Basic Research Program of China. Grant Number: 2012 CB619101
- Chongqing City. Grant Number: CSTC 2010 AB5116
- Fundamental Research Funds for the Central Universities. Grant Numbers: CDJZR 11230010, CDJXS 11230008
- Public Experiment Center of State Bioindustrial Base, Chongqing, China
In-stent restenosis and subsequent thrombosis remain a significant complication following the implantation of coronary stents. Different approaches have been used in developing novel coronary stents to protect against thrombosis and minimize restenosis. In the present study, we designed a biomacromolecular layer-by-layer coating with heparin, vascular endothelial growth factor (VEGF), and fibronectin onto nickel-free titanium surface to improve blood compatibility and endothelial cell proliferation. The multilayer assembling process was monitored by water contact angle and surface plasmon resonance, respectively. With increasing the number of layers, the deposition of polyelectrolyte as self-assembled ultrathin multilayer films showed linear growth of absorbance. In vitro blood compatibility results revealed that the fabricated layers prolonged activated partial thrombin time and prothrombin time, reduced platelets activation and aggregation, and reduced blood hemolysis rate. Cell adhesion and growth results showed that the assembled multilayer films significantly promoted cell attachment and growth, and the endothelialization property of the multilayer films was preferable compared with the untreated titanium disk. In conclusion, these results suggest that titanium surface modification using biofunctional multilayer films composed of heparin, VEGF, and fibronectin may serve as a potential approach to inhibit thrombosis and promote re-endothelialization of cardiovascular stents. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.